Difference between revisions of "Vincent 2015 Front Physiol"
| (11 intermediate revisions by 3 users not shown) | |||
| Line 1: | Line 1: | ||
{{Publication | {{Publication | ||
|title=Vincent G, Lamon S, Gant N, Vincent PJ, MacDonald JR, Markworth JF, Edge JA, Hickey AJ (2015) Changes in mitochondrial function and mitochondria associated protein expression in response to 2-weeks of high intensity interval training. Front Physiol 6:51. | |title=Vincent G, Lamon S, Gant N, Vincent PJ, MacDonald JR, Markworth JF, Edge JA, Hickey AJ (2015) Changes in mitochondrial function and mitochondria associated protein expression in response to 2-weeks of high intensity interval training. Front Physiol 6:51. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/25759671 PMID:25759671] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/25759671 PMID:25759671 Open Access] | ||
|authors=Vincent G, Lamon S, Gant N, Vincent PJ, MacDonald JR, Markworth JF, Edge JA, Hickey AJ | |authors=Vincent G, Lamon S, Gant N, Vincent PJ, MacDonald JR, Markworth JF, Edge JA, Hickey AJ | ||
|year=2015 | |year=2015 | ||
| Line 9: | Line 9: | ||
Eight males performed a single-leg cycling protocol (12 × 1 min intervals at 120% peak power output, 90 s recovery, 4 days/week). Muscle biopsies (vastus lateralis) were taken pre- and post-HIT. Mitochondrial respiration in permeabilized fibers, citrate synthase (CS) activity and protein expression of peroxisome proliferator-activated receptor gamma coactivator (PGC-1α) and respiratory complex components were measured. | Eight males performed a single-leg cycling protocol (12 × 1 min intervals at 120% peak power output, 90 s recovery, 4 days/week). Muscle biopsies (vastus lateralis) were taken pre- and post-HIT. Mitochondrial respiration in permeabilized fibers, citrate synthase (CS) activity and protein expression of peroxisome proliferator-activated receptor gamma coactivator (PGC-1α) and respiratory complex components were measured. | ||
HIT training improved peak power and time to fatigue. Increases in absolute oxidative phosphorylation (OXPHOS) capacities and CS activity were observed, but not in the ratio of | HIT training improved peak power and time to fatigue. Increases in absolute oxidative phosphorylation (OXPHOS) capacities and CS activity were observed, but not in the ratio of CIV to the electron transport system (CIV/ETS), the respiratory control ratios (RCR-1 and RCR-2) or mitochondrial-associated protein expression. Specific increases in OXPHOS flux were not apparent after normalization to CS, indicating that gross changes mainly resulted from increased mitochondrial mass. | ||
Over only 2 weeks HIT significantly increased mitochondrial function in skeletal muscle independently of detectable changes in mitochondrial-associated and mitogenic protein expression. | Over only 2 weeks HIT significantly increased mitochondrial function in skeletal muscle independently of detectable changes in mitochondrial-associated and mitogenic protein expression. | ||
|keywords=HIT, PGC-1α, mitochondria, oxidative phosphorylation, skeletal muscle | |keywords=HIT, PGC-1α, mitochondria, oxidative phosphorylation, skeletal muscle | ||
|mipnetlab=NZ Auckland Hickey AJ | |mipnetlab=NZ Auckland Hickey AJ | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
| Line 20: | Line 20: | ||
|tissues=Skeletal muscle | |tissues=Skeletal muscle | ||
|preparations=Permeabilized tissue | |preparations=Permeabilized tissue | ||
|couplingstates=LEAK, OXPHOS, | |enzymes=Complex IV;cytochrome c oxidase | ||
| | |couplingstates=LEAK, OXPHOS, ET | ||
|pathways=N, S, ROX | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional= | |additional=MiR05, MitoEAGLE muscle buffer, | ||
}} | }} | ||
[[Image:Logo MitoFit.jpg|right|120px|link=http://www.mitofit.org/index.php/MitoFit|MitoFit]] | |||
== MitoFit news 2015#8 == | |||
* 2015-06-16: HIT and fit: 2-weeks of high intensity interval training. »[[K-Regio_MitoFit#2015 |MitoFit news]] | |||
== Correction == | == Correction == | ||
An | :::: An Oroboros Oxygraph-2k was used in this publication, whereas the Anton Paar/Oroboros Oxygraph was the first-generation instrument for high-resolution respirometry, which was replaced by the O2k in 2002. | ||
''Further details'' | |||
:* [[Gnaiger 2012 Abstract Bioblast-Gentle Science]] | |||
:* Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301:R1078–87. [[Pesta 2011 Am J Physiol Regul Integr Comp Physiol |»Bioblast link«]] | |||
Latest revision as of 21:50, 8 March 2020
| Vincent G, Lamon S, Gant N, Vincent PJ, MacDonald JR, Markworth JF, Edge JA, Hickey AJ (2015) Changes in mitochondrial function and mitochondria associated protein expression in response to 2-weeks of high intensity interval training. Front Physiol 6:51. |
Vincent G, Lamon S, Gant N, Vincent PJ, MacDonald JR, Markworth JF, Edge JA, Hickey AJ (2015) Front Physiol
Abstract: High-intensity short-duration interval training (HIT) stimulates functional and metabolic adaptation in skeletal muscle, but the influence of HIT on mitochondrial function remains poorly studied in humans. Mitochondrial metabolism as well as mitochondrial-associated protein expression were tested in untrained participants performing HIT over a 2-week period.
Eight males performed a single-leg cycling protocol (12 × 1 min intervals at 120% peak power output, 90 s recovery, 4 days/week). Muscle biopsies (vastus lateralis) were taken pre- and post-HIT. Mitochondrial respiration in permeabilized fibers, citrate synthase (CS) activity and protein expression of peroxisome proliferator-activated receptor gamma coactivator (PGC-1α) and respiratory complex components were measured.
HIT training improved peak power and time to fatigue. Increases in absolute oxidative phosphorylation (OXPHOS) capacities and CS activity were observed, but not in the ratio of CIV to the electron transport system (CIV/ETS), the respiratory control ratios (RCR-1 and RCR-2) or mitochondrial-associated protein expression. Specific increases in OXPHOS flux were not apparent after normalization to CS, indicating that gross changes mainly resulted from increased mitochondrial mass.
Over only 2 weeks HIT significantly increased mitochondrial function in skeletal muscle independently of detectable changes in mitochondrial-associated and mitogenic protein expression. • Keywords: HIT, PGC-1α, mitochondria, oxidative phosphorylation, skeletal muscle
• O2k-Network Lab: NZ Auckland Hickey AJ
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style
Organism: Human
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Enzyme: Complex IV;cytochrome c oxidase
Coupling state: LEAK, OXPHOS, ET Pathway: N, S, ROX HRR: Oxygraph-2k
MiR05, MitoEAGLE muscle buffer
MitoFit news 2015#8
- 2015-06-16: HIT and fit: 2-weeks of high intensity interval training. »MitoFit news
Correction
- An Oroboros Oxygraph-2k was used in this publication, whereas the Anton Paar/Oroboros Oxygraph was the first-generation instrument for high-resolution respirometry, which was replaced by the O2k in 2002.
Further details
- Gnaiger 2012 Abstract Bioblast-Gentle Science
- Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301:R1078–87. »Bioblast link«
