Layout for DatLab graphs

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MitoPedia

Layout for DatLab graphs

Description

A Layout in DatLab from the Layout menu or the Graph menu is a predefined selection for standardized display of graphs, plots to be displayed at specific scalings. Any defined graph layout can be selected from the Layout menu for defining initial settings for the plots [Ctrl+F6] and scaling [F6]. A layout can be modified and saved under a specific name.


MitoPedia O2k and high-resolution respirometry: DatLab 



Graph / Info

Graph-menu.png

Graph info

View and edit information, and load or save a graph layout. (See below
A quick selection of the tabs Plots Ctrl+F6 and Scaling (F6) is possible.

Layout: Info/ Load/ Save

Layout-menu.png
Open this window (Section above) to view and edit information, and load or save a graph layout.
Info load and save
Types - Four types of layout can be selected depending on the O2k-configuration:
  • O2: Oxygen channel ony.
  • O2 & Amp: Oxygen and amperometric channel.
  • O2 & pX: Oxygen and potentiometric channel.
  • Other


Standard layouts - O2k-FluoRespirometer

Version: DatLab 7
In typical standard DatLab graph layouts plots are shown for the left chamber in Graph 1 (top), and for the right chamber in Graph 2 (below). During data acquisition, a 30 min time range is frequently used and can be adjusted any time during the experiment [F6].


Summary
01 Calibration show Temp
02 Calibration - Background
03 Background high O2
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04a Flux per volume
04b Flux per volume overlay
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05a Specific flux
05b Specific flux overlay
06a Specific flux high O2
06b Specific flux high O2 overlay
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07a Flux Control Ratios
07b Flux Control Ratios overlay

01 Calibration show Temp

General information
Layouts 1 ff. are considered for O2k applications.
Layouts Amp* or pX* are selected for O2k-MultiSensor applications.
Calibration show temp shows O2 concentration and the negative slope in Graph 1 and 2, and temperature and Peltier power in Graph 3: This is the default layout used after switching on the O2k for performing oxygen calibration.
Graph 1 & 2: O2 concentration (blue lines, left Y-axis) and O2 slope negative (uncorrected; red lines, right Y-axis) are displayed in the top graph for the left chamber (A), and below for the right chamber (B).
Graph 3: The third graph (bottom) shows the block temperature on the left Y axis (red) and the Peltier power on the right Y axis (green). Only when both temperature and Peltier power are constant, the chambers have reached thermal equilibrium.
  1. During this phase a stirrer test can be performed [F9].
  2. The next step is to observe equilibration of the oxygen signal with a defined gas phase above the stirred aqueous phase ('open') chamber; usually with air as the first step to perform an oxygen sensor calibration.
» Further information: O2 calibration - DatLab

02 Calibration - Background

Is used for recording O2 sensor calibration and instrumental O2 background test. 'O2 slope neg.' is the negative slope of oxygen concentration, multiplied by 1000 to convert to units [pmol/ml], over time [s]. No correction is applied for instrumental O2 background flux, J°02. 'O2 slope neg.' is plotted on the right Y-axis with a scaling to display ±10 pmol∙s-1∙ml-1 and zero in the middle of the Y2 axis. Zero slope in the 'open' chamber at air calibration indicates stability of the oxygen signal. After closing the chamber, the slope deviates from zero as a function of the oxygen consumption of the polarographic oxygen sensor and of oxygen diffusion into or out of the chamber, which is the first point in the instrumental O2 background test.
» Further information: MiPNet14.06 Instrumental O2 background

03 Background high O2

Is used for recording an instrumental O2 background test at high oxygen from 150 to 450 µM.

04a Flux per volume

It displays background-corrected oxygen flux per volume, which is most relevant to evaluate experimental details, i.e. flux per volume is optimally in the range of 20 to 200-500 pmol∙s-1∙ml-1. This plot is also chosen when measurements on sample density are available only at a later stage. Total O2 flux is corrected for instrumental O2 background, J°O2,V, to obtain sample oxygen flux per chamber volume, JO2,V(sample):
JO2,V(sample) = JO2,V(total) - J°O2,V

04b Flux per volume overlay

It is similar to the layout 04a Flux per volume. Graph 1 shows the background-corrected oxygen flux per volume [pmol∙s-1∙ml-1] superimposed from both chambers. Graph 2 shows oxygen concentration [µM].

05a Specific flux

Is used for plotting background-corrected oxygen flux per unit sample. The unit as a marker for the amount of sample is defined in the F3 window. Example: Select Unit mg for the amount (mass) of sample added to the chamber. The mass-concentration is automatically calculated (division by chamber volume, typically 2 ml). Then mass-specific flux [pmol∙s-1∙mg-1] is displayed as volume-specific flux [pmol∙s-1∙ml-1] divided by mass-concentration [mg/ml]:
JO2,mass = JO2,V / mass-concentration

05b Specific flux overlay

It is similar to ‘05a Specific flux’. Graph 1 shows the background-corrected oxygen flux per unit sample superimposed from both chambers. Graph 2 shows oxygen concentration [µM].

06a Specific flux high O2

This is the same as ‘05a Specific flux’ with the scaling adapted to the high oxygen concentration used for permeabilized muscle fibers (150 to 450 µM; range 300 µM).

06b Specific flux high 02 overlay

As ‘06a Specific flux high O2’, but oxygen flux (graph 1) and oxygen concentration (graph 2) superimposed from both chambers.

07a Flux control ratios

It shows the flux control ratio (FCR) and the oxygen concentration for the left chamber in graph 1 and for the right chamber in graph 2. First, the reference and baseline metabolic states are marked and the marks are named (Marks \ Names). Then the marks are selected in the menu Flux/Slope \ O2 slope.

07b Flux control ratios overlay

As ‘07a Flux control ratios’, but FCR (graph 1) and oxygen concentration (graph 2) superimposed from both chambers.


Lab layouts

A Standard layout or any other layout can be modified and saved under Lab layouts, which is recommended for a team using project-specific layouts.

User: Name

A Standard layout or any other layout can be modified and saved under a specific user name, which is recommended to distinguish individual layouts from standard layouts used by a team.


Reference Layouts

Reference Layouts: O2 & Amp

Version: DatLab 7
01 Amp Amperometric_Raw signal
02 Amp Amperometric_Calibrated


Reference Layouts: O2 & pX

Version: DatLab 7
01 Potentiometric
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02a TPP_calibration
02b TPP_with_O2flux
02c TPP_calibrated_with_O2flux


Reference Layouts: Other

Version: DatLab 7
O2 raw signal