Palmitoylcarnitine

high-resolution terminology - matching measurements at high-resolution

Palmitoylcarnitine

Description

Palmitoylcarnitine is an ester derivative of carnitine (long-chain acylcarnitine) involved in the metabolism of fatty acids. Within the cell, palmitoylcarnitine is transported into the mitochondria to deliver palmitate for fatty acid oxidation and energy production.

Abbreviation: Pal

Reference: Cecatto 2023 MitoFit

Carnitine palmitoyltransferase I (CPT1) also known as carnitine acyltransferase I or CAT1 is a mitochondrial enzyme. CPT1 is associated with the outer mitochondrial membrane and mediates the transport of long-chain fatty acids across the membrane by binding them to carnitine.

Application in HRR

Pal: Palmitoylcarnitine (Palmitoyl-DL-carnitine-HCl; C₂₃H₄₆NO₄ · Cl), Sigma-Aldrich: P4509, store at -20 °C, CAS: 6865-14-1, M = 436.07 g·mol^-1

Preparation of 10 mM stock solution (dissolved in H₂O):

1. Weigh 8.72 mg of palmitoylcarnitine.
2. Dissolve in 2 mL H₂O.
3. Divide into 0.2 mL portions.
4. Store frozen at -20 °C.

» O2k manual titrations MiPNet09.12 O2k-Titrations

• Titration volume: 2−8 µL using a 10 µL syringe (2 mL O2k-chamber).
• Final concentration: 10−40 µM. We suggest testing for the optimum concentration for your sample, as palmitoylcarnitine may inhibit respiration (Cecatto 2023 MitoFit).

MitoPedia topics: Substrate and metabolite 

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MitoPedia:FAT4BRAIN