Pham 2014 Am J Physiol
|Pham T, Loiselle D, Power A, Hickey AJ (2014) Mitochondrial inefficiencies and anoxic ATP hydrolysis capacities in diabetic rat heart. Am J Physiol 307:C499–507.|
Abstract: As approximately 80% of diabetics die from heart failure, understanding diabetic cardiomyopathy is crucial. Mitochondria occupy 35-40% of mammalian cardiomyocyte volume, supply 95% of the hearts' ATP, and diabetic heart mitochondria show impaired structure, arrangement and function. We predict that bioenergetic efficiencies are present in diabetic heart mitochondria; therefore we explored mitochondrial proton and electron handling by linking oxygen flux within streptozotocin (STZ)-induced-diabetic Sprague-Dawley rat heart tissues, to steady-state ATP synthesis, Reactive Oxygen Species (ROS) production, and mitochondrial membrane potential (ΔΨ). By coupling high-resolution respirometers with purpose-built fluorometers, we followed Magnesium Green (ATP synthesis), Amplex Ultra Red (ROS production), and safranin-O (ΔΨ). Relative to control rats, the mass-specific respiration of STZ-diabetic hearts was depressed in oxidative phosphorylating (OXPHOS) states. Steady-state ATP synthesis capacity was almost a third lower in STZ-diabetic heart and relative to O2 flux, this equates to an estimated 12% depression in OXPHOS efficiency. However, with anoxic transition, STZ-diabetic and control heart tissues showed similar ATP hydrolysis capacities through reversal of the F1/F0 ATP-synthase. STZ-diabetic cardiac mitochondria also produced more net ROS relative to oxygen flux (ROS/O) in OXP. While ΔΨ did not differ between groups, the time to develop ΔΨ with the onset of OXPHOS was protracted in STZ-diabetic mitochondria. ROS/O is higher in life-like OXPHOS states and potential delays in the time to develop ΔΨ may delay ATP synthesis with inter-beat fluctuations in ADP concentrations. Whereas diabetic cardiac mitochondria produce less ATP in normoxia, they consume as much ATP in anoxic infarct-like states.
• Keywords: Diabetic cardiomypathy, Anoxia, Efficiency, Mitochondria, Oxidative phosphorylation, Safranin, Amplex Red, Magnesium Green
• O2k-Network Lab: NZ Auckland Hickey AJ
Labels: MiParea: Respiration, mt-Medicine Pathology: Diabetes, Myopathy Stress:Ischemia-reperfusion, Oxidative stress;RONS, Mitochondrial disease Organism: Rat Tissue;cell: Heart Preparation: Homogenate
Regulation: Coupling efficiency;uncoupling, mt-Membrane potential Coupling state: LEAK, OXPHOS, ET Pathway: N, NS, ROX HRR: Oxygraph-2k, O2k-Fluorometer
Safranin, MgG, AmR
Demonstration of using Amplex Red, Safranin and Magnesium green methods with the O2k-Fluoresececen Module.
An Oroboros Oxygraph-2k was used in this publication, whereas the Anton Paar/Oroboros Oxygraph was the first-generation instrument for high-resolution respirometry, which was replaced by the Oxygraph-2k in 2002.
- Further details: Gnaiger 2012 Abstract Bioblast-Gentle Science