Difference between revisions of "2-Deoxyglucose"

Revision as of 11:14, 21 June 2021

high-resolution terminology - matching measurements at high-resolution

2-Deoxyglucose

Description

2-Deoxyglucose, also known as 2-deoxy-D-glucose is a glucose derivative that has the 2-hydroxyl group replaced by hydrogen. It competitively inhibits glycolysis by blocking hexokinase and phosphohexoseisomerase.

Abbreviation: 2-DG

Reference: Proton_flux, O2k-pH_ISE-Module

Application in HRR

2-DG: 2-deoxy-D-glucose C₆H₁₂0₅); Sigma D6134, 1 g or 5 g, store at 2-8 °C; M = 164.16 g·mol^-1

Preparation of 2 M stock solution (dissolved in H₂O):

weigh 1g of 2-deoxy-D-glucose
dissolve 3.0458 mL H₂O in a 10 mL-glass beaker

»O2k manual titrations: MiPNet09.12 O2k-Titrations

Titration volume: 25 µL using a 50 µL syringe
Final conc. in 2 mL O2k-chamber: 25 mM

MitoPedia topics: Inhibitor

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=2-DG
-|description='''2-Deoxyglucose''', also known as 2-deoxy-D-glucose is a glucose derivative which has the 2-hydroxyl group replaced by hydrogen. It competitively inhibits glycolysis by blocking hexokinase and phosphohexoseisomerase .
+|description='''2-Deoxyglucose''', also known as 2-deoxy-D-glucose is a glucose derivative that has the 2-hydroxyl group replaced by hydrogen. It competitively inhibits glycolysis by blocking hexokinase and phosphohexoseisomerase.
 |info=[[Proton_flux]], [[O2k-pH_ISE-Module]]
 }}