Difference between revisions of "Antimycin A"
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|info=[[MiPNet09.12]] | |info=[[MiPNet09.12]] | ||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
{{Chemical_description | |||
|abbr=Ama | |||
|trivial name=Antimycin A | |||
|complete name=from ''Streptomyces'' sp. | |||
|chem formula= | |||
|molar mass=540 | |||
|vendor=Sigma-Aldrich | |||
|product number=A8674 | |||
|store at=-20 °C | |||
|sensitivity= | |||
|cas=1397-94-0 | |||
|h statements=H300, H400 | |||
::: '''Ama: Antimycin A''' (from ''Streptomyces sp.'') Sigma A 8674, 25 mg, store at -20 °C: M = 540 g·mol<sup>-1</sup> | |h info=fatal if swallowed, very toxic to aquatic life}}<!--::: '''Ama: Antimycin A''' (from ''Streptomyces sp.'') Sigma A 8674, 25 mg, store at -20 °C: M = 540 g·mol<sup>-1</sup>--> | ||
:::: Sigma recommends to store desiccated, there is no hint to light sensitivity. | |||
:::: <span style="color:#8B008B"> '''Caution:''' '''Very toxic.''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | :::: Light sensitivity of Antimycin A is reported by another vendor: [https://www.alfa.com/en/catalog/J63522/ AlfaAesar]. | ||
<!--:::: <span style="color:#8B008B"> '''Caution:''' '''Very toxic.''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening. Ama is hygroscopic.</span>--> | |||
:::: '''Preparation of 5 mM stock solution''' (dissolved in | :::: '''Preparation of 5 mM stock solution''' (dissolved in ethanol abs.) for '''2-mL O2k-chamber''': | ||
::::# Weigh 5.4 mg of antimycin A into a small glass vial | ::::# Weigh 5.4 mg of antimycin A into a small glass vial. | ||
::::# Add 2 mL ethanol. | ::::# Add 2 mL ethanol. | ||
::::# Divide into 0.2 mL portions in clear glass vials. | ::::# Divide into 0.2 mL portions in clear glass vials. | ||
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:::: '''Preparation of 1 | :::: '''Preparation of 1.25 mM stock solution''' (dissolved in ethanol abs.) for '''0.5-mL O2k-chamber''': | ||
::::# Take 100 µL of the 5 mM stock solution. | ::::# Take 100 µL of the 5 mM stock solution. | ||
| Line 40: | Line 55: | ||
:::'''Microalgae applications''' | :::'''Microalgae applications''' | ||
::: For the specific case of microalgal cells it is recommended to prepare higher concentration: | ::: For the specific case of microalgal cells it is recommended to prepare higher concentration: | ||
:::: '''Preparation of 10 mM stock solution''' (dissolved in | :::: '''Preparation of 10 mM stock solution''' (dissolved in ethanol abs.) | ||
::::# Weigh 10.8 mg of antimycin A and dissolve in 2 mL of ethanol | ::::# Weigh 10.8 mg of antimycin A and dissolve in 2 mL of ethanol abs.. | ||
::::# Divide into 0.2 mL portions in clear glass vials. | ::::# Divide into 0.2 mL portions in clear glass vials. | ||
::::# Store at -20 °C. | ::::# Store at -20 °C. | ||
| Line 63: | Line 78: | ||
::::* Vamecq J, Schepers L, Parmentier G, Mannaerts GP (1987) Inhibition of peroxisomal fatty acyl-CoA oxidase by antimycin A. Biochem J 248:603-7. - [[Vamecq 1987 Biochem J |»Bioblast link«]] | ::::* Vamecq J, Schepers L, Parmentier G, Mannaerts GP (1987) Inhibition of peroxisomal fatty acyl-CoA oxidase by antimycin A. Biochem J 248:603-7. - [[Vamecq 1987 Biochem J |»Bioblast link«]] | ||
{{MitoPedia concepts}} | |||
{{MitoPedia methods}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Inhibitor | |mitopedia topic=Inhibitor | ||
}} | }} | ||
Latest revision as of 13:23, 15 March 2022
- high-resolution terminology - matching measurements at high-resolution
Antimycin A
Description
Antimycin A is an inhibitor of Complex III (CIII). It binds to the Qi site of CIII and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor). High concentrations of antimycin A also inhibit acyl-CoA oxidase and D-amino acid oxidase.
Abbreviation: Ama
Reference: MiPNet09.12
Application in HRR
- Ama: Antimycin A (from Streptomyces sp.; ), Sigma-Aldrich: A8674, store at -20 °C, CAS: 1397-94-0, M = 540 g·mol-1
- Hazard statements: H300, H400; fatal if swallowed, very toxic to aquatic life
- Ama: Antimycin A (from Streptomyces sp.; ), Sigma-Aldrich: A8674, store at -20 °C, CAS: 1397-94-0, M = 540 g·mol-1
- Sigma recommends to store desiccated, there is no hint to light sensitivity.
- Light sensitivity of Antimycin A is reported by another vendor: AlfaAesar.
- Preparation of 5 mM stock solution (dissolved in ethanol abs.) for 2-mL O2k-chamber:
- Weigh 5.4 mg of antimycin A into a small glass vial.
- Add 2 mL ethanol.
- Divide into 0.2 mL portions in clear glass vials.
- Store at -20 °C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
- Final concentration: 2.5 µM.
- Preparation of 1.25 mM stock solution (dissolved in ethanol abs.) for 0.5-mL O2k-chamber:
- Take 100 µL of the 5 mM stock solution.
- Add 300 µL ethanol.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
- Final concentration: 2.5 µM.
- Microalgae applications
- For the specific case of microalgal cells it is recommended to prepare higher concentration:
- Preparation of 10 mM stock solution (dissolved in ethanol abs.)
- Weigh 10.8 mg of antimycin A and dissolve in 2 mL of ethanol abs..
- Divide into 0.2 mL portions in clear glass vials.
- Store at -20 °C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 8 µL using a 10 µL syringe.
- Final concentration: 40 µM.
Compare
- Myxothiazol - this CIII inhibitor induces less ROS production but is more expensive than Ama.
Antimycin A and ROS production
- Is hydrogen peroxide production measured after inhibition of the Electron transfer-pathway by rotenone and antimycin A of diagnostic significance? - Please, share your evidence (Email to erich.gnaiger@oroboros.at).
Inhibition of acyl-CoA oxidase and D-amino acid oxidase
- Vamecq J, Schepers L, Parmentier G, Mannaerts GP (1987) Inhibition of peroxisomal fatty acyl-CoA oxidase by antimycin A. Biochem J 248:603-7. - »Bioblast link«
MitoPedia topics:
Inhibitor
