Difference between revisions of "Antimycin A"
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::: '''Ama: Antimycin A''' (from ''Streptomyces sp.'') Sigma A 8674, 25 mg, store at -20 °C: M = 540 g·mol<sup>-1</sup> | ::: '''Ama: Antimycin A''' (from ''Streptomyces sp.'') Sigma A 8674, 25 mg, store at -20 °C: M = 540 g·mol<sup>-1</sup> | ||
:::: Sigma recommends to store dessicated, there is no hint to light sensitivity. | |||
:::: <span style="color:#8B008B"> '''Caution:''' '''Very toxic.''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | :::: Light sensitivity of Antimycin A is reported by another vendor: [https://www.alfa.com/en/catalog/J63522/ AlfaAesar]. | ||
:::: <span style="color:#8B008B"> '''Caution:''' '''Very toxic.''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening. Ama is hygroscopic.</span> | |||
:::: '''Preparation of 5 mM stock solution''' (dissolved in 99.9% ethanol) for '''2-mL O2k-chamber''': | :::: '''Preparation of 5 mM stock solution''' (dissolved in 99.9% ethanol) for '''2-mL O2k-chamber''': | ||
::::# Weigh 5.4 mg of antimycin A into a small glass vial | ::::# Weigh 5.4 mg of antimycin A into a small glass vial. | ||
::::# Add 2 mL ethanol. | ::::# Add 2 mL ethanol. | ||
::::# Divide into 0.2 mL portions in | ::::# Divide into 0.2 mL portions in glass vials. | ||
::::# Store at -20 °C. | ::::# Store at -20 °C. | ||
Revision as of 11:53, 16 March 2021
Description
Antimycin A is an inhibitor of Complex III (CIII). It binds to the Qi site of CIII and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor). High concentrations of antimycin A also inhibit acyl-CoA oxidase and D-amino acid oxidase.
Abbreviation: Ama
Reference: MiPNet09.12
Application in HRR
- Ama: Antimycin A (from Streptomyces sp.) Sigma A 8674, 25 mg, store at -20 °C: M = 540 g·mol-1
- Sigma recommends to store dessicated, there is no hint to light sensitivity.
- Light sensitivity of Antimycin A is reported by another vendor: AlfaAesar.
- Caution: Very toxic. Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening. Ama is hygroscopic.
- Ama: Antimycin A (from Streptomyces sp.) Sigma A 8674, 25 mg, store at -20 °C: M = 540 g·mol-1
- Preparation of 5 mM stock solution (dissolved in 99.9% ethanol) for 2-mL O2k-chamber:
- Weigh 5.4 mg of antimycin A into a small glass vial.
- Add 2 mL ethanol.
- Divide into 0.2 mL portions in glass vials.
- Store at -20 °C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
- Final concentration: 2.5 µM.
- Preparation of 1,25 mM stock solution (dissolved in 99.9% ethanol) for 0.5-mL O2k-chamber:
- Take 100 µL of the 5 mM stock solution.
- Add 300 µL ethanol.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
- Final concentration: 2.5 µM.
- Microalgae applications
- For the specific case of microalgal cells it is recommended to prepare higher concentration:
- Preparation of 10 mM stock solution (dissolved in 99.9% ethanol)
- Weigh 10.8 mg of antimycin A and dissolve in 2 mL of ethanol absolute (99.9%).
- Divide into 0.2 mL portions in clear glass vials.
- Store at -20 °C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 8 µL using a 10 µL syringe.
- Final concentration: 40 µM.
Compare
- Myxothiazol - this CIII inhibitor induces less ROS production but is more expensive than Ama.
Antimycin A and ROS production
- Is hydrogen peroxide production measured after inhibition of the Electron transfer-pathway by rotenone and antimycin A of diagnostic significance? - Please, share your evidence (Email to erich.gnaiger@oroboros.at).
Inhibition of acyl-CoA oxidase and D-amino acid oxidase
- Vamecq J, Schepers L, Parmentier G, Mannaerts GP (1987) Inhibition of peroxisomal fatty acyl-CoA oxidase by antimycin A. Biochem J 248:603-7. - »Bioblast link«
MitoPedia topics:
Inhibitor