Difference between revisions of "Ap5A"
From Bioblast
(refined instructions according to SUIT chemical paper) |
|||
Line 10: | Line 10: | ||
:::: '''Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 °C; molecular weight: 1026.28 | :::: '''Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 °C; molecular weight: 1026.28 | ||
:::: <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | :::: <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | ||
:::: '''Preparation of 100 mM stock solution''' (dissolved in | |||
:::: '''Preparation of 100 mM stock solution''' (dissolved in H<sub>2</sub>O) | |||
::::# Dissolve content of Sigma 2 mg glass vial in 19.49 µL H<sub>2</sub>O | |||
::::# Store at -20 °C. | |||
:::» '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | :::» '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::::* Titration volume: 1 µL using a 10 µL syringe | |||
::::* Titration volume ('''2-mL O2k-chamber'''): 1 µL using a 10 µL Hamilton syringe. | |||
::::* Final concentration: 50 µM. | ::::* Final concentration: 50 µM. | ||
== References == | == References == |
Revision as of 09:15, 17 May 2021
Description
P1,P5-Di(adenosine-5')pentaphosphate (Ap5A) is an inhibitor of adenylate kinase (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP).
Abbreviation: Ap5A
Application in HRR
- Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt Sigma D4022, store at -20 °C; molecular weight: 1026.28
- Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
- Preparation of 100 mM stock solution (dissolved in H2O)
- Dissolve content of Sigma 2 mg glass vial in 19.49 µL H2O
- Store at -20 °C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
- Final concentration: 50 µM.
References
MitoPedia topics: Inhibitor