Difference between revisions of "Atractyloside"
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{{MitoPedia methods|type=Inhibitor PS | {{MitoPedia methods|type=Inhibitor PS | ||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
::: '''Atr: Atractyloside''' (Atractyloside dipotassium salt, C<sub>30</sub>H<sub>44</sub>O<sub>16</sub>S<sub>2</sub>K<sub>2</sub>); Sigma A 6882, 250 mg, store at R.T.; | ::: '''Atr: Atractyloside''' (Atractyloside dipotassium salt, C<sub>30</sub>H<sub>44</sub>O<sub>16</sub>S<sub>2</sub>K<sub>2</sub>); Sigma A 6882, 250 mg, store at R.T.; M = 802.99 g·mol<sup>-1</sup> containing 2.5 mol H<sub>2</sub>O/mol. | ||
:::: <span style="color:#8B008B"> '''Caution:''' very toxic.</span> | :::: <span style="color:#8B008B"> '''Caution:''' very toxic.</span> | ||
::: '''Preparation of 50 mM stock solution''' (dissolved in H<sub>2</sub>0): | :::: '''Preparation of 50 mM stock solution''' (dissolved in H<sub>2</sub>0): | ||
::::# Weigh 40.2 mg atractyloside. | ::::# Weigh 40.2 mg atractyloside. | ||
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::: '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | :::» '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::::* Titration volume: 30 µl using a 50 µl syringe (2 ml O2k-chamber). | ::::* Titration volume: 30 µl using a 50 µl syringe (2 ml O2k-chamber). | ||
::::* Final concentration: 0.75 mM. | ::::* Final concentration: 0.75 mM. | ||
::::* '''Comment:''' With Atr, a very high concentration is required for full | ::::* '''Comment:''' With Atr, a very high concentration is required for full inhibition. [[carboxyatractyloside|Carboxyatractyloside]] (Cat) is considered to be much more specific than Atr, and the effective concentration required is much much lower. | ||
{{MitoPedia topics | |||
|mitopedia topic=Inhibitor | |||
|type=Inhibitor PS | |||
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Latest revision as of 12:47, 12 February 2021
- high-resolution terminology - matching measurements at high-resolution
Atractyloside
Description
Atractyloside is an inhibitor of the adenine nucleotide translocator (ANT). It is an extremely toxic glycoside that inhibits oxidative phosphorylation by blocking the transfer of adenosine nucleotides through the mitochondrial membrane.
Abbreviation: Atr
Reference: MiPNet03.02; MiPNet09.12
Application in HRR
- Atr: Atractyloside (Atractyloside dipotassium salt, C30H44O16S2K2); Sigma A 6882, 250 mg, store at R.T.; M = 802.99 g·mol-1 containing 2.5 mol H2O/mol.
- Caution: very toxic.
- Preparation of 50 mM stock solution (dissolved in H20):
- Weigh 40.2 mg atractyloside.
- Dissolve in 1 ml H2O (it dissolves better in warm water).
- Divide into 0.2 ml aliquots.
- Store at -20 °C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 30 µl using a 50 µl syringe (2 ml O2k-chamber).
- Final concentration: 0.75 mM.
- Comment: With Atr, a very high concentration is required for full inhibition. Carboxyatractyloside (Cat) is considered to be much more specific than Atr, and the effective concentration required is much much lower.
MitoPedia topics: Inhibitor
