Azide

high-resolution terminology - matching measurements at high-resolution

Azide

Description

Sodium azide is an inhbitor of cytochrome c oxidase (COX, CcO).

Abbreviation: Azd

Reference: MiPNet03.02

Application in HRR

Azd: Azide (Sodum azide; NaN₃), Sigma-Aldrich: S2002, store at RT, CAS: 26628-22-8, M = 65.01 g·mol^-1

Hazard statements: H300, H310, H330, H373, H410; fatal if swallowed, fatal in contact with skin, fatal if inhaled, may cause damage to organs through prolonged or repeated exposure (brain), very toxic to aquatic life with long-lasting effects

2021-03: Preparation instructions were updated to take the volume of the solute into account (see Volume_of_the_solute). The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations.

Preparation of 4 M storage and stock solution (1 mL, dissolved in H₂O) for 2-mL O2k-chamber:

1. Weigh 260 mg of sodium azide.
2. Dissolve in 900 µL H₂0.
3. Divide into 0.5 mL portions.
4. Store at -20 °C.

»O2k manual titrations MiPNet09.12 O2k-Titrations

• Titration volume (2-mL O2k-chamber): ≥50 µL using a 100 µL Hamilton syringe.
• Final concentration: ≥100 mM.

Preparation of 2 M stock solution (dissolved in H₂O) for 0.5-mL O2k-chamber:

1. Take 400 µL of the 4 M stock solution.
2. Add 400 µL H₂O.

»O2k manual titrations MiPNet09.12 O2k-Titrations

• Titration volume (0.5-mL O2k-chamber): ≥25 µL using a 100 µL Hamilton syringe.
• Final concentration: ≥100 mM.

MitoPedia topics: Inhibitor