Difference between revisions of "Cell ergometry"
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|mitopedia topic=Respiratory state | |mitopedia topic=Respiratory state | ||
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[[File:OROBOROS Poster HRR.jpg|960px]] | [[File:OROBOROS Poster HRR.jpg|960px]] | ||
High-resolution pdf: Β»[[File:OROBOROS Poster HRR.pdf]] | High-resolution pdf: Β»[[File:OROBOROS Poster HRR.pdf]] | ||
[[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:E.jpg|link=ETS capacity|ETS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]] | |||
== Figure 1: Phosphorylation control protocol in the intact cell == | == Figure 1: Phosphorylation control protocol in the intact cell == |
Revision as of 18:59, 15 August 2014
Description
Biochemical cell ergometry aims at measurement of JO2,max (compare VO2,max in exercise ergometry of humans and animals) of cell respiration linked to phosphorylation of ADP to ATP. The corresponding OXPHOS capacity is based on saturating concentrations of ADP, [ADP]*, and inorganic phosphate, [Pi]*, available to the mitochondria. This is metabolically opposite to uncoupling respiration, which yields ETS capacity. The OXPHOS state can be established experimentally by selective permeabilization of cell membranes with maintenance of intact mitochondria, titrations of ADP and Pi to evaluate kinetically saturating conditions, and establishing fuel substrate combinations which reconstitute physiological TCA cycle function.
Abbreviation: n.a.
Reference: Gnaiger 2014 MitoPathways
MitoPedia methods:
Respirometry
MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
960px High-resolution pdf: Β»File:OROBOROS Poster HRR.pdf
Figure 1: Phosphorylation control protocol in the intact cell
- Biochemical coupling efficiency: jE-L = (E-L)/E
- ETS excess factor over R: ExR/E = (E-R)/E
- ROUTINE phosphorylation control factor: βR/R = (R-L)/R
- ROUTINE phosphorylation control ratio: βR/E = (R-L)/E