Difference between revisions of "Dinitrophenole"
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== Application in [[ | == Application in [[HRR]] == | ||
::: '''Preparation of 10 mM DNP stock solution''' (dissolved in H<sub>2</sub>O): | ::: '''Preparation of 10 mM DNP stock solution''' (dissolved in H<sub>2</sub>O): | ||
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:::# Weigh 3.7 mg of DNP (FW = 184.11). | :::# Weigh 3.7 mg of DNP (FW = 184.11). | ||
:::# Dissolve in 1.2 | :::# Dissolve in 1.2 mL H<sub>2</sub>O. | ||
:::# Neutralize with 1 M KOH. | :::# Neutralize with 1 M KOH. | ||
:::# Adjust final volume to 2 | :::# Adjust final volume to 2 mL and divide into 0.2 mL portions. | ||
:::# Store at -20 °C. | :::# Store at -20 °C. | ||
Revision as of 13:01, 17 March 2019
- high-resolution terminology - matching measurements at high-resolution
Dinitrophenole
Description
2,4-dinitrophenole (C6H4N2O5; FW = 184.11) is a protonophore acting as an uncoupler of oxidative phosphorylation.
Abbreviation: DNP
Reference: Steinlechner-Maran 1996 Am J Physiol Cell Physiol
- 2,4-dinitrophenole (DNP) is a compound that was designed for the manufacture of munitions during the first world war and lately described in 1933 by Maurice Tainter as a weight loss drug due to its effect on basal metabolic rate. DNP decreases the ADP phosphorylation in mitochondria preventing the uptake of inorganic phosphate into the organelle. At the same time, its characteristics as ionophore stimulates oxygen consumption, increasing proton conductance and heat dissipation.
MitoPedia topics: Uncoupler
Application in HRR
- Preparation of 10 mM DNP stock solution (dissolved in H2O):
- Caution: Toxic!
- Weigh 3.7 mg of DNP (FW = 184.11).
- Dissolve in 1.2 mL H2O.
- Neutralize with 1 M KOH.
- Adjust final volume to 2 mL and divide into 0.2 mL portions.
- Store at -20 °C.
