Difference between revisions of "Fatty acid oxidation"

Revision as of 16:47, 2 September 2014

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Fatty acid oxidation

Description

Fatty acids (short chain with 4–8, medium-chain with 6–12, long chain with 16-22 carbon atoms) are activated by fatty acyl-CoA synthases (thiokinases) in the cytosol. The outer mt-membrane enzyme carnitine palmitoyltransferase 1 (CPT-1) generates an acyl-carnitine intermediate for transport into the mt-matrix. Octanoate and palmitate (eight- and 16-carbon saturated fatty acids) may pass the mt-membranes but are frequently supplied to mt-preparations in the activated form of octanoylcarnitine or palmitoylcarnitine.

Electron-transferring flavoprotein complex (CETF) is located on the matrix face of the inner mt-membrane, and supplies electrons from fatty acid β-oxidation (FAO) to CoQ. FAO cannot proceed without a substrate combination of fatty acids & malate, and inhibition of CI blocks FAO completely. Fatty acids are split stepwise into two carbon fragments forming acetyl-CoA, which enters the TCA cycle by condensation with oxaloacetate (CS reaction). Therefore, FAO implies simultaneous electron transfer into the Q-junction trough CETF and CI.

Abbreviation: FAO

Reference: Gnaiger 2014 MitoPathways

MitoPedia methods: Respirometry

MitoPedia topics: Substrate and metabolite

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 {{MitoPedia
 |abbr=FAO
-|description=Fatty acids (short chain with 4–8, medium-chain with 6–12 carbon atoms) must be activated by fatty acyl-CoA synthases (thiokinases). The outer mt-membrane enzyme carnitine palmitoyltransferase 1 (CPT-1) generates an acyl-carnitine intermediate for transport into the mt-matrix. Therefore, [[octanoate]] and [[palmitate]] (eight- and 16-carbon saturated fatty acids) are frequently supplied to mt-preparations in the activated form of [[octanoylcarnitine]] or [[palmitoylcarnitine]].
+|description=Fatty acids (short chain with 4–8, medium-chain with 6–12, long chain with 16-22 carbon atoms) are activated by fatty acyl-CoA synthases (thiokinases) in the cytosol. The outer mt-membrane enzyme carnitine palmitoyltransferase 1 (CPT-1) generates an acyl-carnitine intermediate for transport into the mt-matrix. [[Octanoate]] and [[palmitate]] (eight- and 16-carbon saturated fatty acids) may pass the mt-membranes but are frequently supplied to mt-preparations in the activated form of [[octanoylcarnitine]] or [[palmitoylcarnitine]].
-Electron-transferring flavoprotein complex (CETF) is located on the matrix face of the inner mt-membrane, and supplies electrons from fatty acid β-oxidation (FAO) to CoQ. FAO cannot proceed without a substrate combination of fatty acids & malate, and inhibition of CI blocks FAO completely. Fatty acids are split stepwise into two carbon fragments forming acetyl-CoA, which enter the TCA cycle by condensation with oxaloacetate (CS reaction). FAO therefore implies simultaneous electron transfer into the Q-junction trough CETF and CI.
+[[Electron-transferring flavoprotein complex]] (CETF) is located on the matrix face of the inner mt-membrane, and supplies electrons from fatty acid β-oxidation (FAO) to CoQ. FAO cannot proceed without a substrate combination of fatty acids & [[malate]], and inhibition of CI blocks FAO completely. Fatty acids are split stepwise into two carbon fragments forming acetyl-CoA, which enters the TCA cycle by condensation with oxaloacetate (CS reaction). Therefore, FAO implies simultaneous electron transfer into the [[Q-junction]] trough CETF and CI.
 |info=Gnaiger 2014 MitoPathways