Difference between revisions of "Fluorescence-Sensor"
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== O2k-Guide == | == O2k-Guide == | ||
=== Select the Fluorescence-Sensors === | === Select the Fluorescence-Sensors === | ||
Switching between different excitation wavelengths and filters is achieved by simply exchanging the fluorescence sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters: | Switching between different excitation wavelengths and filters is achieved by simply exchanging the fluorescence sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters. Select the necessary filter set form the table of [[O2k-Fluorescence_LED2-Module#Application_specific_settings|application specific settings]]. Each fluorescence sensor is deliver with a filter set already in place: | ||
# [[Fluorescence-Sensor Green|LED 525 nm wavelength: Green]]. The installed Filter-Cap is ready for H2O2 measurement with [[Amplex red|Amplex® UltraRed]]. | # [[Fluorescence-Sensor Green|LED 525 nm wavelength: Green]]. The installed Filter-Cap is ready for H2O2 measurement with [[Amplex red|Amplex® UltraRed]]. | ||
# [[Fluorescence-Sensor Blue|LED 465 nm wavelength: Blue]]. The installed Filter-Cap is ready for measurements of mt-membrane potential with [[safranin]]. A different filter is used for measurement with [[Magnesium green| Magnesium green®]] or Calcium green® . | # [[Fluorescence-Sensor Blue|LED 465 nm wavelength: Blue]]. The installed Filter-Cap is ready for measurements of mt-membrane potential with [[safranin]]. A different filter is used for measurement with [[Magnesium green| Magnesium green®]] or Calcium green® . | ||
Revision as of 15:27, 8 May 2012
Fluorescence-Sensor
| Description | Fluorescence-Sensor: LED with specified wavelength, photodiode, Filter-Cap attached with specific optical filter for the LED and/or photodiode. See Fluorescence-Sensor Green and Fluorescence-Sensor Blue. |
|---|---|
| Product ID | 442## |
| Type | O2k, MultiSensor, O2k-Fluorescence LED2-Module |
| Link | O2k-Fluorescence LED2-Module |
| Image |  |
O2k-Guide
Select the Fluorescence-Sensors
Switching between different excitation wavelengths and filters is achieved by simply exchanging the fluorescence sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters. Select the necessary filter set form the table of application specific settings. Each fluorescence sensor is deliver with a filter set already in place:
- LED 525 nm wavelength: Green. The installed Filter-Cap is ready for H2O2 measurement with Amplex® UltraRed.
- LED 465 nm wavelength: Blue. The installed Filter-Cap is ready for measurements of mt-membrane potential with safranin. A different filter is used for measurement with Magnesium green® or Calcium green® .
O2k-Guide: Setup - next step Mounting a Filter-Cap
Connect Fluorescence-Sensor to O2k
For recording of fluorescence signals, insert the black sensor into the window of the O2k chamber as far as possible, aligning the Sensor-Guide Sector with the Sensor-Guide of the O2k-Front Fixation. Then connect the cable of the Fluorescence-Sensor to the Fluorescence-Sensor Plug of the Fluorescence-Control Unit.
Stoppers
Use only black PEEK stoppers in conjunction with fluorometric measurements. If necessary, replace the previous white PVDF stoppers. The black stoppers can be used for all HRR applications in general. See MiPNet12.06 for calibration of the O2k-chamber volume, which is identical for PEEK and PVDF stoppers.
O2k-Guide: Electronic settings
