Difference between revisions of "Glucose"

Revision as of 09:02, 11 September 2020

high-resolution terminology - matching measurements at high-resolution

Glucose

Description

Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.

Abbreviation: Glc

Reference: MiPNet15.09. Yeast: HRR Reference Assay

Application in HRR

Glc: Glucose (D-(+)-Glucose, C₆H₁₂0₆); Sigma G 8270, 100 g, store at RT; FW = 180.16

Preparation of 2 M stock solution (dissolved in H₂O):

weigh 360.32 mg of Glucose
dissolve 1 mL H₂O in 1.5 mL Eppendorf

»O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume: 20 µL using a 50 µL syringe
Final conc. in 2 mL O2k-chamber: 20 mM

Preparation of 2.2 M stock solution (dissolved in H₂O):

weigh 79.2 mg of Glucose
dissolve 0.2 mL H₂O in 0.5 mL Eppendorf

»O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume: 5 µL using a 25 µL syringe
Final conc. in 2 mL O2k-chamber: 5.5 mM (1 g/L)

Titration volume: 10 µL using a 25 µL syringe
Final conc. in 2 mL O2k-chamber: 11 mM (2 g/L)

MitoPedia topics: Substrate and metabolite

@@ Line 4: / Line 4: @@
 |info=[[MiPNet15.09. Yeast: HRR Reference Assay]]
 }}
-{{MitoPedia concepts}}
-{{MitoPedia methods}}
+__TOC__
-{{MitoPedia O2k and high-resolution respirometry}}
-{{MitoPedia topics
-|mitopedia topic=Substrate and metabolite
-}}
 == Application in [[HRR]] ==
-'''Glc:  Glucose''' (D-(+)-Glucose,  C<sub>6</sub>H<sub>12</sub>0<sub>6</sub>);  Sigma G 8270, 100 g, store at RT; FW = 180.16
+:::'''Glc:  Glucose''' (D-(+)-Glucose,  C<sub>6</sub>H<sub>12</sub>0<sub>6</sub>);  Sigma G 8270, 100 g, store at RT; FW = 180.16
-'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O):
+::::'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O):
-::1) weigh 360.32 mg of Glucose
+::::# weigh 360.32 mg of Glucose
-::2) dissolve 1 mL H<sub>2</sub>O in 1.5 mL Eppendorf
+::::# dissolve 1 mL H<sub>2</sub>O in 1.5 mL Eppendorf
-'''Oxygraph-2k Titration:'''
+:::»'''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
-::* Titration volume: 20 µL using a 50 µL syringe
+::::* Titration volume: 20 µL using a 50 µL syringe
-::* Final conc. in 2 mL O2k-chamber: 20 mM
+::::* Final conc. in 2 mL O2k-chamber: 20 mM
-'''Preparation of 2.2 M stock solution''' (dissolved in H<sub>2</sub>O):
+::::'''Preparation of 2.2 M stock solution''' (dissolved in H<sub>2</sub>O):
-::1) weigh 79.2 mg of Glucose
+::::# weigh 79.2 mg of Glucose
-::2) dissolve 0.2 mL H<sub>2</sub>O in 0.5 mL Eppendorf
+::::# dissolve 0.2 mL H<sub>2</sub>O in 0.5 mL Eppendorf
-'''Oxygraph-2k Titration:'''
+:::»'''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
-::* Titration volume: 5 µL using a 25 µL syringe
+::::* Titration volume: 5 µL using a 25 µL syringe
-::* Final conc. in 2 mL O2k-chamber: 5.5 mM (1 g/L)
+::::* Final conc. in 2 mL O2k-chamber: 5.5 mM (1 g/L)
-::* Titration volume: 10 µL using a 25 µL syringe
+::::* Titration volume: 10 µL using a 25 µL syringe
-::* Final conc. in 2 mL O2k-chamber: 11 mM (2 g/L)
+::::* Final conc. in 2 mL O2k-chamber: 11 mM (2 g/L)
+{{MitoPedia concepts}}
+{{MitoPedia methods}}
+{{MitoPedia O2k and high-resolution respirometry}}
+{{MitoPedia topics
+|mitopedia topic=Substrate and metabolite
+}}