Gnaiger 2014 Abstract MiP2014
| Cell ergometry and respiratory control factors: limitation of measurements, terminology and concepts. |
Link:
Gnaiger E (2014)
Event: MiP2014
Biochemical cell ergometry aims at measurement of JO2,max (compare VO2,max in exercise ergometry of humans and animals) of cell respiration linked to phosphorylation of ADP to ATP. The corresponding OXPHOS capacity is based on saturating concentrations of ADP, [ADP]*, and inorganic phosphate, [Pi]*, available to the mitochondria. This is metabolically opposite to uncoupling respiration, which yields ETS capacity. The OXPHOS state can be established experimentally by selective permeabilization of cell membranes with maintenance of intact mitochondria, titrations of ADP and Pi to evaluate kinetically saturating conditions, and establishing fuel substrate combinations which reconstitute physiological TCA cycle function.
Labels: MiParea: Respiration
HRR: Oxygraph-2k Event: A4, Oral MiP2014
Figures
960px High-resolution pdf: »File:OROBOROS Poster HRR.pdf
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Figure 1: Phosphorylation control protocol in the intact cell
- Biochemical coupling efficiency: jE-L = (E-L)/E
- ETS excess factor over R: ExR/E = (E-R)/E
- ROUTINE phosphorylation control factor: ≈R/R = (R-L)/R
- ROUTINE phosphorylation control ratio: ≈R/E = (R-L)/E
