Horseradish peroxidase

high-resolution terminology - matching measurements at high-resolution

Horseradish peroxidase

Description

Horseradish peroxidase readily combines with hydrogen peroxide (H₂O₂) and the resultant [HRP-H₂O₂] complex can oxidize a wide variety of hydrogen donors.

Abbreviation: HRP

MitoPedia methods: Fluorometry 

Application in HRR

HRP: Horseradish peroxidase (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C

Reaction

Donor + H₂O₂ → Oxidized Donor + 2 H₂O

Known Inhibitors

according to Sigma-Adrich, citing : sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd²⁺, Co²⁺, Cu²⁺, Fe³⁺, Mn²⁺, Ni²⁺, and Pb²⁺ ions.

Comment

The use of horseradish peroxidase Typ II in fluorometric measurement of peroxide production was recommended by Laszlo Tretter.