Difference between revisions of "MiPNet06.03 POS-calibration-SOP"

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MiPNet06.03 POS-calibration-SOP

O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.

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OROBOROS (2016-02-09) Mitochondr Physiol Network

Abstract: Gnaiger E (2016) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(15):1-8.

O2k-Protocols SOP: High-resolution respirometry (HRR; OROBOROS Oxygraph-2k, O2k) critically depends on maintenance (MiPNet19.18B POS-Service) and accurate calibration of the polarographic oxygen sensors (OroboPOS, POS). Standard operating procedures (O2k-SOP) are described as part of the MitoFit Quality Control System. (1) Clean and ready-to-use O2k-Chambers are a basic requirement (MiPNet19.03). (2) Quality control for evaluation of proper POS function (SOP: O2 sensor test). (3) Accurate POS calibration (MiPNet19.18D O2k-Calibration). This is part 1 of O2k Quality Control.

» O2k-Protocols SOP: O2k Quality Control 2 »MiPNet14.06 InstrumentalO2Background«

» O2k-Manual »MiPNet19.18D_O2k-Calibration«

» Product: OroboPOS, O2k, O2k-Catalogue

» Template: O2k Calibration List

• O2k-Network Lab: AT_Innsbruck_OROBOROS

Labels: MiParea: Instruments;methods

HRR: Oxygraph-2k, O2k-Protocol

O2k-SOP

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Further details

Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York:321-33. »Bioblast Access«

Next step - O2k Quality Control 2: »MiPNet14.06 InstrumentalO2Background«

^cO2* is the oxygen concentration at thermodynamic equilibrium with air under standard conditions of 100 kPa barometric pressure. F_M has been determined for MiR05 at 30 °C and 37 °C, and is assumed to be identical at 25 °C.

References

This article is cited in

Rodriguez JV, Pizarro MD, Scandizzi AL, Guibert EE, Almada LL, Mamprin ME (2008) Construction and performance of a minibioreactor suitable as experimental bioartificial liver. Artif Organs 32:323-8. »PubMed 18370948«

@@ Line 1: / Line 1: @@
 {{OROBOROS header page name}}
 {{Publication
-|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] Polarographic oxygen sensors: calibration, accuracy and quality control SOP.
+|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.
 |info=[[File:PDF.jpg|50px|link=http://wiki.oroboros.at/images/7/77/MiPNet06.03_POS-Calibration-SOP.pdf |Bioblast pdf]] » [http://www.bioblast.at/index.php/File:MiPNet06.03_POS-Calibration-SOP.pdf Versions]
 |authors=OROBOROS
-|year=2016-02-07
+|year=2016-02-09
 |journal=Mitochondr Physiol Network
-|abstract=Gnaiger E (2016) Polarographic oxygen sensors: calibration, accuracy and quality control SOP. Mitochondr Physiol Network 06.03(15):1-8.
+|abstract=Gnaiger E (2016) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(15):1-8.
-[[High-resolution respirometry]] critically depends on accurate calibration of the [[polarographic oxygen sensor]]s which respond to [[Oxygen_pressure| partial oxygen pressure]].  Expressing the oxygen signal in terms of partial pressure has several advantages, but respiration is expressed in molar units related to biochemical stoichiometries. Conversion of oxygen partial pressure to oxygen concentration requires accurate information on oxygen solubilities in experimental incubation media. Absolute errors up to 10% are commonly found in the established literature. The [[oxygen solubility]] of mitochondrial respiration medium [[MiR06]] relative to pure water (oxygen solubility factor, ''F''<sub>M</sub>) is 0.92, accurately determined (for MiR05, i.e. MiR06 without catalase; [[MiPNet14.13]]) at 37 °C and 30 °C.  At air saturation at standard barometric pressure (100 kPa) and 37 °C, the partial oxygen pressure is 19.63 kPa, and the oxygen concentration is 190.7 µM in MiR06.  A clean and ready-to-use O2k-Chamber is a requirement for saving time and quickly calibrating the polarographic oxygen sensors.
+'''[[O2k-SOP |O2k-Protocols SOP]]''': [[High-resolution respirometry]] (HRR; OROBOROS Oxygraph-2k, O2k) critically depends on maintenance ([[MiPNet19.18B POS-Service]]) and accurate calibration of the [[polarographic oxygen sensor]]s ([[OroboPOS]], POS). Standard operating procedures (O2k-SOP) are described as part of the [[MitoFit Quality Control System]]. (1) Clean and ready-to-use O2k-Chambers are a basic requirement ([[MiPNet19.03]]). (2) Quality control for evaluation of proper POS function (SOP: O2 sensor test). (3) Accurate POS calibration ([[MiPNet19.18D O2k-Calibration]]). This is part 1 of O2k Quality Control.
-:» O2k-Manual: »[[MiPNet19.18D_O2k-Calibration]]«
+:» O2k-Protocols SOP: O2k Quality Control 2 »[[MiPNet14.06 InstrumentalO2Background]]«
+:» O2k-Manual »[[MiPNet19.18D_O2k-Calibration]]«
 :» Product: [[OroboPOS]], [[Oxygraph-2k|O2k]], [[OROBOROS O2k-Catalogue |O2k-Catalogue]]
 :» Template: [[File:O2k-Calibration-List.xls|O2k Calibration List]]
@@ Line 19: / Line 21: @@
 |additional=O2k-SOP
 }}
+__TOC__
 {{Technical support integrated}}
 [[File:O2 sensor test.jpg|right|400px|link=O2 sensor test |O2 sensor test]]
-__TOC__
+== Further details ==
-== Further details ==
-* Next step of instrumental quality control: »[[MiPNet14.06 InstrumentalO2Background]]«
 * Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York:321-33. [[Forstner_1983_POS |»Bioblast Access«]]
+[[File:O2k-QCS.jpg|350px|O2k-QCS]] Next step - O2k Quality Control 2: »[[MiPNet14.06 InstrumentalO2Background]]«