Difference between revisions of "MiPNet06.03 POS-calibration-SOP"
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|abstract='''Gnaiger E (2016) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(15):1-8.''' » [http://www.bioblast.at/index.php/File:MiPNet06.03_POS-Calibration-SOP.pdf Versions] | |abstract='''Gnaiger E (2016) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(15):1-8.''' » [http://www.bioblast.at/index.php/File:MiPNet06.03_POS-Calibration-SOP.pdf Versions] | ||
'''[[O2k-SOP |O2k-Protocols SOP]]''': [[High-resolution respirometry]] (HRR; OROBOROS Oxygraph-2k, O2k) critically depends on maintenance ([[MiPNet19.18B POS-Service]]) and accurate calibration of the [[polarographic oxygen sensor]]s ([[OroboPOS]], POS). Standard operating procedures (O2k-SOP) are described as part of the [[MitoFit Quality Control System]]. (1) Clean and ready-to-use O2k-Chambers are a basic requirement ([[MiPNet19.03]]). (2) Quality control for evaluation of proper POS function (SOP: O2 sensor test). (3) Accurate POS calibration ([[MiPNet19.18D O2k- | '''[[O2k-SOP |O2k-Protocols SOP]]''': [[High-resolution respirometry]] (HRR; OROBOROS Oxygraph-2k, O2k) critically depends on maintenance ([[MiPNet19.18B POS-Service]]) and accurate calibration of the [[polarographic oxygen sensor]]s ([[OroboPOS]], POS). Standard operating procedures (O2k-SOP) are described as part of the [[MitoFit Quality Control System]]. (1) Clean and ready-to-use O2k-Chambers are a basic requirement ([[MiPNet19.03]]). (2) Quality control for evaluation of proper POS function (SOP: O2 sensor test). (3) Accurate POS calibration ([[MiPNet19.18D O2k-calibration]]). This is part 1 of O2k Quality Control. | ||
:» O2k-Protocols SOP: O2k Quality Control 2 »[[MiPNet14.06 InstrumentalO2Background]]« | :» O2k-Protocols SOP: O2k Quality Control 2 »[[MiPNet14.06 InstrumentalO2Background]]« | ||
:» O2k-Manual »[[MiPNet19.18D_O2k- | :» O2k-Manual »[[MiPNet19.18D_O2k-calibration]]« | ||
:» Product: [[OroboPOS]], [[Oxygraph-2k|O2k]], [[OROBOROS O2k-Catalogue |O2k-Catalogue]] | :» Product: [[OroboPOS]], [[Oxygraph-2k|O2k]], [[OROBOROS O2k-Catalogue |O2k-Catalogue]] | ||
:» Template: [[File:O2-calibration.xlsx|O2-calibration.xlsx]] | :» Template: [[File:O2-calibration.xlsx|O2-calibration.xlsx]] | ||
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::::* Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York:321-33. - [[Forstner_1983_POS |»Bioblast link«]] | ::::* Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York:321-33. - [[Forstner_1983_POS |»Bioblast link«]] | ||
::::* [[Gnaiger_2008_POS]] | ::::* [[Gnaiger_2008_POS]] | ||
::::* [[MiPNet19.18D_O2k- | ::::* [[MiPNet19.18D_O2k-calibration]] | ||
::: '''This article is cited in''' | ::: '''This article is cited in''' | ||
Revision as of 09:18, 14 August 2016
MiPNet06.03 POS-calibration-SOP
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» 
OROBOROS (2016-02-09) Mitochondr Physiol Network
Abstract: Gnaiger E (2016) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(15):1-8. » Versions
O2k-Protocols SOP: High-resolution respirometry (HRR; OROBOROS Oxygraph-2k, O2k) critically depends on maintenance (MiPNet19.18B POS-Service) and accurate calibration of the polarographic oxygen sensors (OroboPOS, POS). Standard operating procedures (O2k-SOP) are described as part of the MitoFit Quality Control System. (1) Clean and ready-to-use O2k-Chambers are a basic requirement (MiPNet19.03). (2) Quality control for evaluation of proper POS function (SOP: O2 sensor test). (3) Accurate POS calibration (MiPNet19.18D O2k-calibration). This is part 1 of O2k Quality Control.
- » O2k-Protocols SOP: O2k Quality Control 2 »MiPNet14.06 InstrumentalO2Background«
- » O2k-Manual »MiPNet19.18D_O2k-calibration«
- » Product: OroboPOS, O2k, O2k-Catalogue
- » Template: O2-calibration.xlsx
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
HRR: Oxygraph-2k, O2k-Protocol
O2k-SOP
Further details
Next step - O2k Quality Control 2: »MiPNet14.06 InstrumentalO2Background«
cO2* is the oxygen concentration at thermodynamic equilibrium with air under standard conditions of 100 kPa barometric pressure. FM has been determined for MiR05 at 30 °C and 37 °C, and is assumed to be identical at 25 °C.
References
- Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York:321-33. - »Bioblast link«
- Gnaiger_2008_POS
- MiPNet19.18D_O2k-calibration
- This article is cited in
- Rodriguez JV, Pizarro MD, Scandizzi AL, Guibert EE, Almada LL, Mamprin ME (2008) Construction and performance of a minibioreactor suitable as experimental bioartificial liver. Artif Organs 32:323-8. »PubMed 18370948«
- This article is cited in
