Difference between revisions of "MiPNet07.01 Advances"
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|title= | |title=Advances in high-resolution respirometry. Mitochondr. Physiol. Network 7.1. | ||
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|year=* | |year=* | ||
|mipnetlab= | |journal=Mitochondr. Physiol. Network | ||
|mipnetlab=AT_Innsbruck_OROBOROS | |||
|abstract=The OROBOROS [[Oxygraph-2k]] with [[DatLab]] 4 software is the sole-source instrument for [[high-resolution respirometry]], with the option of modular [[O2k-MultiSensor]] extension and electronically controlled [[Titration-Injection microPump]] (TIP2k), and accessories including the [[Integrated Suction System]] (ISS) and titration syringes. Please, visit [http://www.oroboros.at OROBOROS] for newest information. | |||
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==Full text== | |||
The OROBOROS O2k-MultiSensor extension is the basis for application of our new Ion Selective Electrode (ISE) system for measurement of pH, mitochondrial membrane potential (TPP+ electrode), and additional electrochemical sensors (NO). Our innovations are at the interface between instrumental development, research in bioenergetics and biomedicine, and world-wide distribution. Whereas the high-resolution analysis of oxygen concentration and flux remains the fundamental basis, the O2k-MultiSensor system integrates specific requirements in mitochondrial physiology with ion-selective electrodes and optical measurements. | |||
Our instruments are represented in leading laboratories for basic and clinical research in 34 countries. You may contact our OROBOROS MiPNet Reference Laboratories in your geographical area: http://www.oroboros.at/index.php?mipnet-labs. | |||
The OROBOROS Oxygraph-2k has become the standard where high resolution counts, at low respiratory activities, in particular for the analysis of cell cultures, muscle biopsies, including human applications, and for measurements at critical low oxygen levels. Combination with the Titration-Injection microPump (TIP2k) allows operation with programmable titration regimes and at quasi steady-states, yielding a new flexibility in experimental design by combining the technical advantages of closed and open systems approaches. These developments lead not merely a system of high quality instruments but distinguish high-resolution respirometry as a method and concept. | |||
On the basis of recent developments and experimental tests, the OROBOROS team is excited to report about significant improvements of high-resolution respirometry with the Oxygraph-2k: Advances in electronics, smoother stirring at higher rates, higher precision of the glass chambers, and higher temperature stability of the Peltier system made possible a significant improvement of signal stability (2008-GnaigerE), beyond the high performance reported previously (2008-GnaigerE). Combined with the software upgrade DatLab 4, this opens up new perspectives for measurements with small samples of muscle fibers, human cells and microbiopsies. | |||
===References=== | |||
*[[Gnaiger_2008_POS|Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352.]] | |||
*[[Gnaiger_2001_RespPhysiol|Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir. Physiol. 128: 277-297.]] | |||
Revision as of 12:04, 3 November 2010
| Advances in high-resolution respirometry. Mitochondr. Physiol. Network 7.1. |
» MiPNet07.01
OROBOROS (
) Mitochondr. Physiol. Network
Abstract: The OROBOROS Oxygraph-2k with DatLab 4 software is the sole-source instrument for high-resolution respirometry, with the option of modular O2k-MultiSensor extension and electronically controlled Titration-Injection microPump (TIP2k), and accessories including the Integrated Suction System (ISS) and titration syringes. Please, visit OROBOROS for newest information.
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels:
HRR: Oxygraph-2k, DatLab Software; Separate Application"DatLab Software; Separate Application" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., Method"Method" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Full text
The OROBOROS O2k-MultiSensor extension is the basis for application of our new Ion Selective Electrode (ISE) system for measurement of pH, mitochondrial membrane potential (TPP+ electrode), and additional electrochemical sensors (NO). Our innovations are at the interface between instrumental development, research in bioenergetics and biomedicine, and world-wide distribution. Whereas the high-resolution analysis of oxygen concentration and flux remains the fundamental basis, the O2k-MultiSensor system integrates specific requirements in mitochondrial physiology with ion-selective electrodes and optical measurements.
Our instruments are represented in leading laboratories for basic and clinical research in 34 countries. You may contact our OROBOROS MiPNet Reference Laboratories in your geographical area: http://www.oroboros.at/index.php?mipnet-labs.
The OROBOROS Oxygraph-2k has become the standard where high resolution counts, at low respiratory activities, in particular for the analysis of cell cultures, muscle biopsies, including human applications, and for measurements at critical low oxygen levels. Combination with the Titration-Injection microPump (TIP2k) allows operation with programmable titration regimes and at quasi steady-states, yielding a new flexibility in experimental design by combining the technical advantages of closed and open systems approaches. These developments lead not merely a system of high quality instruments but distinguish high-resolution respirometry as a method and concept.
On the basis of recent developments and experimental tests, the OROBOROS team is excited to report about significant improvements of high-resolution respirometry with the Oxygraph-2k: Advances in electronics, smoother stirring at higher rates, higher precision of the glass chambers, and higher temperature stability of the Peltier system made possible a significant improvement of signal stability (2008-GnaigerE), beyond the high performance reported previously (2008-GnaigerE). Combined with the software upgrade DatLab 4, this opens up new perspectives for measurements with small samples of muscle fibers, human cells and microbiopsies.
