Difference between revisions of "MiPNet10.04 CellRespiration"

Latest revision as of 12:51, 4 December 2019

MiPNet10.04 CellRespiration

An experiment with high-resolution respirometry: coupling control in cell respiration.

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Oroboros (2016-08-08) Mitochondr Physiol Network

Abstract: Doerrier C, Gnaiger E (2005-2016) An experiment with high-resolution respirometry: Coupling control in cell respiration. Mitochondr Physiol Network 10.04(09):1-12.

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• O2k-Network Lab: AT Innsbruck Oroboros

Labels: MiParea: Respiration, Instruments;methods

Organism: Mouse Tissue;cell: Blood cells Preparation: Intact cells

Regulation: Coupling efficiency;uncoupling, Uncoupler Coupling state: LEAK, ROUTINE, ET Pathway: ROX HRR: Oxygraph-2k, TIP2k, O2k-Protocol

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+{{OROBOROS header page name}}
 {{Publication
-|title=Garedew A, Haffner B, Huetter E, Gnaiger E. An experiment with high-resolution respirometry: Phosphorylation control in cell respiration. Mitochondr. Physiol. Network 10.04.
-|authors=Garedew A, Haffner B, Huetter E, Gnaiger E
+|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] An experiment with high-resolution respirometry: coupling control in cell respiration.
-|year=*
+|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/a/a6/MiPNet10.04_CellRespiration.pdf |Bioblast pdf]]  »[http://www.bioblast.at/index.php/File:MiPNet10.04_CellRespiration.pdf Versions]
-|mipnetlab=AT_Innsbruck_OROBOROS
+|authors=Oroboros
-|abstract=Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with cultured, suspended cells in the OROBOROS
+|year=2016-08-08
-Oxygraph-2k (O2k). The experiment demonstrates manual titrations of inhibitors, and automatic titrations of an uncoupler using the electronic Titration-Injection microPump TIP2k. Application of the DatLab 4.3 (upgraded) software is shown for instrumental control (O2k and TIP2k), and on-line data analysis. The following guideline describes the experiment in the form of a laboratory protocol, complementary to the relevant sections of the Oxygraph-2k Manual. The experiments were carried out by participants of an O2k-Course on high-resolution respirometry in April 2005 (IOC30; Schröcken, Austria).
+|journal=Mitochondr Physiol Network
-|info=[http://www.oroboros.at/index.php?id=protocols_cell_hrr_pc MiPNet10.04]
+|abstract=Doerrier C, Gnaiger E (2005-2016) An experiment with high-resolution respirometry: Coupling control in cell respiration. Mitochondr Physiol Network 10.04(09):1-12.
+{{MiPNet pdf page linking to MitoPedia}}
+:» Product: [[Oroboros O2k]], [[Oroboros O2k-Catalogue | O2k-Catalogue]]
+|mipnetlab=AT Innsbruck Oroboros
 }}
 {{Labeling
-|discipline=Mitochondrial Physiology
+|area=Respiration, Instruments;methods
 |organism=Mouse
-|tissues=Blood Cell; Suspension Culture
+|tissues=Blood cells
-|preparations=Intact Cell; Cultured; Primary
+|preparations=Intact cells
-|kinetics=Inhibitor; Uncoupler
+|topics=Coupling efficiency;uncoupling, Uncoupler
-|topics=Respiration; OXPHOS; ETS Capacity, Coupling; Membrane Potential
+|couplingstates=LEAK, ROUTINE, ET
-|instruments=Oxygraph-2k, TIP2k, DatLab Software; Separate Application, Method
+|pathways=ROX
-|articletype=Protocol; Manual, MiPNet-online Publication
+|instruments=Oxygraph-2k, TIP2k, O2k-Protocol
 }}
-[[Category:OroboroPedia]]