MiPNet23.02 IOC127 Szeged HU

MiPNet23.02 IOC127 Szeged HU

Szeged HU, 2018 Mar 27 Oroboros satellite O2k-Workshop on hypoxia IOC127.

Oroboros (2018-03-27) Mitochondr Physiol Network

Abstract:

Oroboros O2k-Workshop on hypoxia in cooperation with HU Szeged Boros M. Szeged, Hungary, 2018 March 27.

• O2k-Network Lab: AT_Innsbruck_OROBOROS, HU Szeged Boros M

General information

This O2k-Workshop takes place satellite to the WISe3 Conference in Szeged, HU. Separate registration is required for this workshop.

Venue

Clinical Skills Centre, Faculty of Medicine

University of Szeged

13 Dom square, HU-6720

Szeged, Hungary

HU Szeged Boros M

Programme

» Will be uploaded shortly.

Local organizer

Prof. Boros Mihaly , HU Szeged Boros M

Registration

Registration will be opened soon.

Tutors and Lecturers

Gnaiger Erich, CEO, Oroboros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory
András Mészáros, MD., PhD., Chief Research Officer, Oroboros Instruments and Assistant Professor, University of Szeged, Institute of Surgical Research
Timea Komlòdi, Scientific assistant, Oroboros Instruments

List of participants

Will be updated

WISe3 Conference Szeged

» WISe3 Conference

Contact

instruments@oroboros.at

Oroboros Instruments

High-Resolution FluoRespirometry

Schöpfstrasse 18

A-6020 Innsbruck, Austria

Tel: +43 512 566796

Fax: +43 512 566796 20

Mitochondria and cell research

IOC recommended reading

» IOC recommended reading

Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: Facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662: 7-25. - »Bioblast link«

Isolated mitochondria and intact cells.
Nanomolar resolution of oxygen concentration.
Effects of cell density and activity.
Closed versus open systems.
p₅₀ in vivo.

Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. - »Bioblast link«

A detailed introduction into high-resolution respirometry with particular emphasis on kinetics and measurements at low oxygen
Mitochondrial kinetics measured by high-resolution respirometry
Calibrations and corrections for response time and instrumental background
Steady-state injection respirometry
Mitochondrial respiratory control at low oxygen
Apparent oxygen affinity and catalytic efficiency of mitochondrial respiration
Effect of ADP and oxygen limitation on ADP/O2 flux ratios
The low-oxygen environment of the cell: Mitochondria between hypoxic and oxidative stress

Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. - »Bioblast link«

First publication to establish high-resolution respirometry, based on the OROBOROS Oxygraph.
Standardized oxygen calibration (air and internal zero oxygen calibration).
Automatic instrumental background correction of oxygen flux.
Deconvolution of the oxygen signal for time resolution required in oxygen kinetics.
Quality control of the limit of detection of oxygen flux at ±1 pmol O₂·s^-1·ml^-1.

Labels:

HRR: Oxygraph-2k

ORO, IOC, O2k-Network, 2018, Next

O2k-Workshops are listed as MitoGlobal Events.'