MiPNet23.11 IOC137 Innsbruck AT: Difference between revisions

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== Programme ==
::::* Programme not available yet: will be uploaded shortly.


== General information ==
== General information ==
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== Registration ==
== Registration ==
::::will open soon
::::will open soon
== Tutors, lecturers, and managers ==
<br/>
<gallery mode=default perrow=6 widths="140px" heights="150px">
File:DoerrierC.JPG|'''[[Doerrier C|Carolina Doerrier]]''', PhD., Oroboros Instruments - Chief scientific officer
File:KomlodiT.JPG|'''[[Komlodi T|Timea KomlΓ²di]]''', PhD., Oroboros Instruments - Scientific researcher
File:Bastos Sant'Anna Silva AC.jpg|'''[[Bastos Sant'Anna Silva AC|Ana Carolina Bastos Sant'Anna Silva]]''', MSc., Oroboros Instruments, PhD. student TRANSMITΒ 
File:Di MarcelloM.jpg|'''[[Di Marcello M|Marco di Marcello]]''', BSc., Medical University of Innsbruck, MitoFit project - Assistant
File:Gnaiger Erich.jpg|'''[[Gnaiger E|Erich Gnaiger]]''', PhD., Oroboros Instruments, CEO
</gallery>
== Programme ==
::::{| class='wikitable'
|-
|style="width: 10em"|'''Monday 10th'''
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|08:30
|OroboPOS service and O2k instrumental setup - overview with videoclips
|ORO-MitoFit Lab
|-
|09:30
|'''Hands-on''': OroboPOS service and O2k instrumental setup
|ORO-MitoFit Lab
|-
|10:10
|'''Hands-on''': DatLab 7
|ORO-MitoFit Lab
|-
|10:30
|''Coffee''
|
|-
|11:00
|Instrumental quality control 1: oxygen calibration
|ORO-MitoFit Lab
|-
|11:30
|'''Hands-on''': Instrumental quality control 1: oxygen calibration
DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
|ORO-MitoFit Lab
|-
|12:00
|''Lunch break''
|
|-
|13:00
|'''Hands-on''':Instrumental quality control 1: oxygen calibration (continuation)
DL-Protocol: O2 calibration air
|ORO-MitoFit Lab
|-
|13:30
|Instrumental quality control 2: Instrumental O2 background – overview with videoclips
|ORO-MitoFit Lab
|-
|14:00
|'''Hands-on''':Instrumental quality control 2: Instrumental O2 background
DL-Protocol: Instrumental O2 background TiP2k
|ORO-MitoFit Lab
|-
|15:00
|''Coffee''
|
|-
|15:30-18:00
|'''Hands-on''': Instrumental quality control 1 and 2. DatLab analysis.
|ORO-MitoFit Lab
|-
|}
::::{| class='wikitable'
|-
|style="width: 10em"|'''Tuesday 11th'''
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|09:00
|Hands-on: Instrumental quality control 1: oxygen calibration
DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
|ORO-MitoFit Lab
|-
|09:15
|Application of the O2k Series HΒ 
|ORO-MitoFit Lab
|-
|09:30
|Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2
|ORO-MitoFit Lab
|-
|10:00
|''Coffee''
|
|-
|10:30
|'''Hands-on''': O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.
DL-Protocol: SUIT-1_O2_pce_D03.DLP
DL-Protocol: SUIT-2 O2 pce D07.DLP
|ORO-MitoFit Lab
|-
|11:45
|'''Hands-on''': O2k-cleaning after use
DL-Protocol: O2k-cleaning after use
|ORO-MitoFit Lab
|-
|12:00
|''Lunch break''
|
|-
|13:00
|'''Hands-on''': O2k-cleaning after use
DL-Protocol: O2k-cleaning after use
|ORO-MitoFit Lab
|-
|13:30
|Introduction to DatLab analysis
|ORO-MitoFit Lab
|-
|15:00
|''Coffee''
|
|-
|15:30-18:00
|Individual DatLab analysis + summary
|ORO-MitoFit Lab
|-
|}
::::{| class='wikitable'
|-
|style="width: 10em"|'''Wednesday 12th'''
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|09:00
|'''MitoFit proficiency test:'''
'''Hands-on''': Instrumental quality control 1: oxygen calibration
DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
|ORO-MitoFit Lab
|-
|10:00
|''Coffee''
|
|-
|10:30
|'''MitoFit proficiency test:'''
'''Hands-on''': O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.
DL-Protocol: SUIT-1_O2_pce_D03.DLP
DL-Protocol: SUIT-2 O2 pce D07.DLP
|ORO-MitoFit Lab
|-
|11:45
|'''Hands-on''': O2k-cleaning after use
DL-Protocol: O2k-cleaning after use
|ORO-MitoFit Lab
|-
|12:00
|Lunch break
|
|-
|13:00
|'''Hands-on''': O2k-cleaning after use
DL-Protocol: O2k-cleaning after use
|ORO-MitoFit Lab
|-
|13:15
|DatLab analysis
|ORO-MitoFit Lab
|-
|14:00
|DatLab analysis summary
|ORO-MitoFit Lab
|-
|15:00
|''Coffee''
|
|-
|15:30
|The Bioblast wiki, O2k-Network and MITOEAGLE
|MiP''Art''
|-
|16:15
|Feedback & conclusions
|MiP''Art''
|-
|17:00
|Farewell activity
|MiP''Art''
|-
|}
== Participants ==
{| class="wikitable"
|-
!
! Participant
! Institution
|-
| [[File:Profile-icon-9.png|70px|Participant]]
| [[Saharnaz Sarlak]]
| University of Bordeaux, Bordeaux (FR)
|-
| [[File:Bastos Sant'Anna Silva AC.jpg|70px|Bastos Sant'Anna Silva AC| ]]
| [[Bastos Sant'Anna Silva AC|Bastos Sant'Anna Silva Ana Carolina]]
| '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT)
|-
| [[File:Profile-icon-9.png|70px|Participant]]
| [[Di Paola Floriana Jessica]]
| Justus-Liebig-University of Giessen, Giessen (DE)
|-
| [[File:Profile-icon-9.png|70px|Participant]]
| [[Thapa Maheshwor]]
| Biocrates Life Sciences AG, Innsbruck (AT)
|-
| [[File:Profile-icon-9.png|70px|Participant]]
| [[Abla Houda]]
| University of Bologna, Bologna (IT)
|-
|}




Revision as of 09:46, 10 September 2018

                

MiPNet23.11 IOC137 Innsbruck AT

Publications in the MiPMap
OROBOROS-solo.png

Innsbruck AT, 2018 Dec 10-12. MitoFit Coaching Days - Basic IOC.


Oroboros (2018-12-10) Mitochondr Physiol Network

Abstract:

O2k-Workshops.png

MitoFit Coaching Days - Basic. Innsbruck, Austria; 2018 Dec 10-12.


β€’ O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit


Labels:






ORO, IOC, 2018, MitoFit, TRANSMIT, Next, MitoGlobal 




General information

MitoFit Coaching Days - Basic provide a fundamental introduction to High-Resolution FluoRespirometry with the Oroboros O2k. Hands-on sessions range from OroboPOS technical service to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
TRANSMIT
Early stage researchers (ESRΒ΄s) during their Marie SkΕ‚odowska-Curie project - TRANSMIT will be trained at the O2k-Workshop on High-Resolution FluoRespirometry.
Β» Information on travel and venue: IOC Innsbruck.
Please bring your labcoat with you. Thank you!

Registration

will open soon

Tutors, lecturers, and managers


Programme

Monday 10th Topic Room
08:30 OroboPOS service and O2k instrumental setup - overview with videoclips ORO-MitoFit Lab
09:30 Hands-on: OroboPOS service and O2k instrumental setup ORO-MitoFit Lab
10:10 Hands-on: DatLab 7 ORO-MitoFit Lab
10:30 Coffee
11:00 Instrumental quality control 1: oxygen calibration ORO-MitoFit Lab
11:30 Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning before use

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
12:00 Lunch break
13:00 Hands-on:Instrumental quality control 1: oxygen calibration (continuation)

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
13:30 Instrumental quality control 2: Instrumental O2 background – overview with videoclips ORO-MitoFit Lab
14:00 Hands-on:Instrumental quality control 2: Instrumental O2 background

DL-Protocol: Instrumental O2 background TiP2k

ORO-MitoFit Lab
15:00 Coffee
15:30-18:00 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. ORO-MitoFit Lab
Tuesday 11th Topic Room
09:00 Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning before use

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
09:15 Application of the O2k Series H ORO-MitoFit Lab
09:30 Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2 ORO-MitoFit Lab
10:00 Coffee
10:30 Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.

DL-Protocol: SUIT-1_O2_pce_D03.DLP

DL-Protocol: SUIT-2 O2 pce D07.DLP

ORO-MitoFit Lab
11:45 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
12:00 Lunch break
13:00 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
13:30 Introduction to DatLab analysis ORO-MitoFit Lab
15:00 Coffee
15:30-18:00 Individual DatLab analysis + summary ORO-MitoFit Lab
Wednesday 12th Topic Room
09:00 MitoFit proficiency test:

Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning before use

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
10:00 Coffee
10:30 MitoFit proficiency test:

Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.

DL-Protocol: SUIT-1_O2_pce_D03.DLP

DL-Protocol: SUIT-2 O2 pce D07.DLP

ORO-MitoFit Lab
11:45 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
12:00 Lunch break
13:00 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
13:15 DatLab analysis ORO-MitoFit Lab
14:00 DatLab analysis summary ORO-MitoFit Lab
15:00 Coffee
15:30 The Bioblast wiki, O2k-Network and MITOEAGLE MiPArt
16:15 Feedback & conclusions MiPArt
17:00 Farewell activity MiPArt

Participants

Participant Institution
Participant Saharnaz Sarlak University of Bordeaux, Bordeaux (FR)
Bastos Sant'Anna Silva AC.jpg Bastos Sant'Anna Silva Ana Carolina AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT)
Participant Di Paola Floriana Jessica Justus-Liebig-University of Giessen, Giessen (DE)
Participant Thapa Maheshwor Biocrates Life Sciences AG, Innsbruck (AT)
Participant Abla Houda University of Bologna, Bologna (IT)


Contact

instruments@oroboros.at


Oroboros Instruments
High-Resolution FluoRespirometry
SchΓΆpfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Mitochondria and cell research

Project support

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
Sponsors.png

IOC recommended reading

Β» IOC recommended reading
Β» MitoPedia: Respiratory states
OXPHOS ROUTINE ETS LEAK ROX


MitoGlobal
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.
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