Difference between revisions of "O2k-Fluo LED2-Module"
From Bioblast
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|description=The '''O2k-Fluorescence Module''' is a modular extension of the [[Oxygraph-2k]], adding a new dimension to [[HRR]]. Optical probes are inserted through the front window of the O2k-glass chambers, for measurement of '''hydrogen peroxide production''' ([[Amplex red]]), '''ATP production''' ([[Mg green]]), '''mt-membrane potential''' ([[Safranin]]), '''Ca<sup>2+</sup>''' ([[Ca green]]), and numerous other applications open for O2k-user innovation. | |description=The '''O2k-Fluorescence Module''' is a modular extension of the [[Oxygraph-2k]], adding a new dimension to [[HRR]]. Optical probes are inserted through the front window of the O2k-glass chambers, for measurement of '''hydrogen peroxide production''' ([[Amplex red]]), '''ATP production''' ([[Mg green]]), '''mt-membrane potential''' ([[Safranin]]), '''Ca<sup>2+</sup>''' ([[Ca green]]), and numerous other applications open for O2k-user innovation. | ||
The O2k-Fluorescence Module can be applied as an add-on to the [[Oxygraph-2k Core | The O2k-Fluorescence Module can be applied as an add-on to the [[Oxygraph-2k Core]], as one of the modules of the [[O2k-MultiSensor System]]. | ||
|product id=34000-01 | |product id=34000-01 | ||
|product type=MultiSensor, Accessory, DatLab, Stopper, Workshop | |product type=MultiSensor, Accessory, DatLab, Stopper, Workshop | ||
|info=http://www.oroboros.at/ | |info=http://www.oroboros.at/?O2k-Fluorescence | ||
}} | }} | ||
{{MitoPedia methods | {{MitoPedia methods | ||
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== Components == | == Components == | ||
The O2k-Fluorescence Module consists of optical probes for both O2k-chambers (green and blue excitation), optical filters, front fixation of the optical probes to the glass windows of the O2k-chambers, electronic control of light intensity, data input into the [[Oxygraph-2k Core | The O2k-Fluorescence Module consists of optical probes for both O2k-chambers (green and blue excitation), optical filters, front fixation of the optical probes to the glass windows of the O2k-chambers, electronic control of light intensity, data input into the [[Oxygraph-2k Core]], and the new '''DatLab''' software. | ||
: 2 Fluorescence sensors, green and blue emission with photodiode and filters | : 2 Fluorescence sensors, green and blue emission with photodiode and filters | ||
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== Upgrading the Oxygraph-2k Basic == | == Upgrading the Oxygraph-2k Basic == | ||
*The [[Oxygraph-2k Core | *The [[Oxygraph-2k Core]] does not require any upgrading, and fully supports the O2k-Fluorescence Module. | ||
*The Oxygraph-2k 20000-44 (Series E), or O2k (Series A-D) requires the O2k-[[MultiSensor|MultiSensor electronic upgrading]]. The [[O2k-MultiSensor System]] fully supports the O2k-Fluorescence Module. Two [[MultiSensor_stopper_PEEK_black_conical,_central_port|black PEEK stoppers]] (with single central titration port) are included in the O2k-Fluorescence Module. | *The Oxygraph-2k 20000-44 (Series E), or O2k (Series A-D) requires the O2k-[[MultiSensor|MultiSensor electronic upgrading]]. The [[O2k-MultiSensor System]] fully supports the O2k-Fluorescence Module. Two [[MultiSensor_stopper_PEEK_black_conical,_central_port|black PEEK stoppers]] (with single central titration port) are included in the O2k-Fluorescence Module. | ||
Revision as of 10:13, 7 January 2012
O2k-Fluo LED2-Module
| Description | The O2k-Fluorescence Module is a modular extension of the Oxygraph-2k, adding a new dimension to HRR. Optical probes are inserted through the front window of the O2k-glass chambers, for measurement of hydrogen peroxide production (Amplex red), ATP production (Mg green), mt-membrane potential (Safranin), Ca2+ (Ca green), and numerous other applications open for O2k-user innovation.
The O2k-Fluorescence Module can be applied as an add-on to the Oxygraph-2k Core, as one of the modules of the O2k-MultiSensor System. |
|---|---|
| Product ID | 34000-01 |
| Type | MultiSensor, Accessory"Accessory" is not in the list (O2k, O2k-Module, O2k-Core, O2k-Fluorometer, O2k-Main Unit, O2k-Assembly Kit, OroboPOS, OroboPOS-Service Kit, ISS, TIP2k, ...) of allowed values for the "Product type" property., DatLab"DatLab" is not in the list (O2k, O2k-Module, O2k-Core, O2k-Fluorometer, O2k-Main Unit, O2k-Assembly Kit, OroboPOS, OroboPOS-Service Kit, ISS, TIP2k, ...) of allowed values for the "Product type" property., Stopper, Workshop"Workshop" is not in the list (O2k, O2k-Module, O2k-Core, O2k-Fluorometer, O2k-Main Unit, O2k-Assembly Kit, OroboPOS, OroboPOS-Service Kit, ISS, TIP2k, ...) of allowed values for the "Product type" property. |
| Link | http://www.oroboros.at/?O2k-Fluorescence |
| Image |
MitoPedia methods:
Fluorometry
Components
The O2k-Fluorescence Module consists of optical probes for both O2k-chambers (green and blue excitation), optical filters, front fixation of the optical probes to the glass windows of the O2k-chambers, electronic control of light intensity, data input into the Oxygraph-2k Core, and the new DatLab software.
- 2 Fluorescence sensors, green and blue emission with photodiode and filters
- 1 Electronic Current Control for regulation of light intensity
- 1 Front Fixation for fluorescence sensors to O2k-glass chamber
- 1 Registration fee waived for the O2k-Fluorometry Workshop.
References
- Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2011) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J. Comp. Physiol. B.[Epub ahead of print].
- Fasching M, Harrison DK, Tretter L, Gnaiger E (2011) Combination of high-resolution respirometry and fluorometry for continuous monitoring of hydrogen peroxide production by mitochondria with resolution in the nanomolar range. Abstract Berlin.
- Sumbalova Z, Harrison DK, Gradl P, Fasching M, Gnaiger E (2011) Mitochondrial membrane potential, coupling control, H2O2 production, and the upper limit of mitochondrial performance. Abstract Kagoshima.
News
The first series of the O2k-Fluorescence Module will be completed by beginning of April 2012, and will be delivered in April 2012, for orders received by end of January 2012.
O2k-Fluorometry Workshop
- O2k-Fluorometry Workshop from 15 to 16 March 2012, Innsbruck, Austria.
Upgrading the Oxygraph-2k Basic
- The Oxygraph-2k Core does not require any upgrading, and fully supports the O2k-Fluorescence Module.
- The Oxygraph-2k 20000-44 (Series E), or O2k (Series A-D) requires the O2k-MultiSensor electronic upgrading. The O2k-MultiSensor System fully supports the O2k-Fluorescence Module. Two black PEEK stoppers (with single central titration port) are included in the O2k-Fluorescence Module.
