Difference between revisions of "O2k-Fluo LED2-Module"
From Bioblast
(Series D+: two sensors per chamber, Series A to C: different info; one chambe only) |
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== Components: O2k-Fluorescence Module== | == Components: O2k-Fluorescence Module== | ||
The O2k-Fluorescence Module consists of optical probes for both O2k-chambers (green and blue excitation), optical filters, front fixation of the optical probes to the glass windows of the O2k-chambers, electronic control of light intensity, data input into the [[O2k-Main Unit]], and the new '''DatLab''' software. | The O2k-Fluorescence Module for O2k Series D and above consists of optical probes for both O2k-chambers (green and blue excitation), optical filters, front fixation of the optical probes to the glass windows of the O2k-chambers, electronic control of light intensity, data input into the [[O2k-Main Unit]], and the new '''DatLab''' software. | ||
: 2 Fluorescence sensors, green and blue emission with photodiode and filters | : 2 Fluorescence sensors per chamber, green and blue emission with photodiode and filters | ||
: 1 Electronic Current Control for regulation of light intensity | : 1 Electronic Current Control for regulation of light intensity | ||
: 1 Front Fixation for fluorescence sensors to O2k-glass chamber | : 1 Front Fixation for fluorescence sensors to O2k-glass chamber | ||
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*[[Hickey 2011 JCPB|Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2011) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J. Comp. Physiol. B.[Epub ahead of print].]] | *[[Hickey 2011 JCPB|Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2011) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J. Comp. Physiol. B.[Epub ahead of print].]] | ||
*[[Hunter 2012 Biochem Pharmacol|Hunter FW, Wanga J, Patela R, Hsua H-L, Hickey AJ, Haya MP, Wilson WR (2012) Homologous recombination repair-dependent cytotoxicity of the benzotriazine di-N-oxide CEN-209: Comparison with other hypoxia-activated prodrugs. Biochem Pharmacol 83: 574–585.]] | |||
*[[Fasching 2011 Abstract Berlin|Fasching M, Harrison DK, Tretter L, Gnaiger E (2011) Combination of high-resolution respirometry and fluorometry for continuous monitoring of hydrogen peroxide production by mitochondria with resolution in the nanomolar range. Abstract Berlin]]. | *[[Fasching 2011 Abstract Berlin|Fasching M, Harrison DK, Tretter L, Gnaiger E (2011) Combination of high-resolution respirometry and fluorometry for continuous monitoring of hydrogen peroxide production by mitochondria with resolution in the nanomolar range. Abstract Berlin]]. | ||
*[[Sumbalova 2011 Abstract Kagoshima|Sumbalova Z, Harrison DK, Gradl P, Fasching M, Gnaiger E (2011) Mitochondrial membrane potential, coupling control, H<sub>2</sub>O<sub>2</sub> production, and the upper limit of mitochondrial performance. Abstract Kagoshima]]. | *[[Sumbalova 2011 Abstract Kagoshima|Sumbalova Z, Harrison DK, Gradl P, Fasching M, Gnaiger E (2011) Mitochondrial membrane potential, coupling control, H<sub>2</sub>O<sub>2</sub> production, and the upper limit of mitochondrial performance. Abstract Kagoshima]]. | ||
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*The [[O2k-Core]] fully supports the O2k-Fluorescence Module. | *The [[O2k-Core]] fully supports the O2k-Fluorescence Module. | ||
*The Oxygraph-2k 20000-44 (Series E | *The Oxygraph-2k 20000-44 (Series D and E) requires the O2k-[[MultiSensor|MultiSensor electronic upgrading]]. The [[O2k-MultiSensor System]] fully supports the O2k-Fluorescence Module. Two [[MultiSensor_stopper_PEEK_black_conical,_central_port|black PEEK stoppers]] (with single central titration port) are included in the O2k-Fluorescence Module. | ||
*Oxygraph-2k 20000-44 (Series A to C). For best performance we recommend to use an O2k Series D and above. However, O2k (Series A to C) can be equipped with a multisensor module upon request. Only one chamber can be used when measuring simlutaneously fluoresecence and respiatin. The other chamber can not be used for any measuremts during fluorecsence experiment, but both chambers can still be used for standard repisration experiments (no fluoresecence). An O2k-[[MultiSensor|MultiSensor electronic upgrading]] is NOT required for O2k (Series A to C). The modified O2k-Fluorescence Module will include fluorescence Sensors for only one chamber and only one [[MultiSensor_stopper_PEEK_black_conical,_central_port|black PEEK stoppers]] (with single central titration port). Additionally, a special pre-amplification box will be included. | |||
Revision as of 12:16, 25 January 2012
O2k-Fluo LED2-Module
| Description | The O2k-Fluorescence Module is a modular extension of the O2k-Core, adding a new dimension to HRR. Optical probes are inserted through the front window of the O2k-glass chambers, for measurement of hydrogen peroxide production (Amplex red), ATP production (Mg green), mt-membrane potential (Safranin), Ca2+ (Ca green), and numerous other applications open for O2k-user innovation. |
|---|---|
| Product ID | 12100-01 |
| Type | O2k, O2k-Module, MultiSensor, Fluorescence Module"Fluorescence Module" is not in the list (O2k, O2k-Module, O2k-Core, O2k-Fluorometer, O2k-Main Unit, O2k-Assembly Kit, OroboPOS, OroboPOS-Service Kit, ISS, TIP2k, ...) of allowed values for the "Product type" property. |
| Link | @OROBOROS, Oxygraph-2k |
| Image |
MitoPedia methods:
Fluorometry
Components: O2k-Fluorescence Module
The O2k-Fluorescence Module for O2k Series D and above consists of optical probes for both O2k-chambers (green and blue excitation), optical filters, front fixation of the optical probes to the glass windows of the O2k-chambers, electronic control of light intensity, data input into the O2k-Main Unit, and the new DatLab software.
- 2 Fluorescence sensors per chamber, green and blue emission with photodiode and filters
- 1 Electronic Current Control for regulation of light intensity
- 1 Front Fixation for fluorescence sensors to O2k-glass chamber
- 1 Registration fee waived for the O2k-Fluorometry Workshop.
References
- Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2011) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J. Comp. Physiol. B.[Epub ahead of print].
- Hunter FW, Wanga J, Patela R, Hsua H-L, Hickey AJ, Haya MP, Wilson WR (2012) Homologous recombination repair-dependent cytotoxicity of the benzotriazine di-N-oxide CEN-209: Comparison with other hypoxia-activated prodrugs. Biochem Pharmacol 83: 574–585.
- Fasching M, Harrison DK, Tretter L, Gnaiger E (2011) Combination of high-resolution respirometry and fluorometry for continuous monitoring of hydrogen peroxide production by mitochondria with resolution in the nanomolar range. Abstract Berlin.
- Sumbalova Z, Harrison DK, Gradl P, Fasching M, Gnaiger E (2011) Mitochondrial membrane potential, coupling control, H2O2 production, and the upper limit of mitochondrial performance. Abstract Kagoshima.
News
The first series of the O2k-Fluorescence Module will be completed by beginning of April 2012, and will be delivered in April 2012, for orders received by end of January 2012.
O2k-Fluorometry Workshop
- O2k-Fluorometry Workshop from 15 to 16 March 2012, Innsbruck, Austria.
Upgrading the Oxygraph-2k Basic
- The O2k-Core fully supports the O2k-Fluorescence Module.
- The Oxygraph-2k 20000-44 (Series D and E) requires the O2k-MultiSensor electronic upgrading. The O2k-MultiSensor System fully supports the O2k-Fluorescence Module. Two black PEEK stoppers (with single central titration port) are included in the O2k-Fluorescence Module.
- Oxygraph-2k 20000-44 (Series A to C). For best performance we recommend to use an O2k Series D and above. However, O2k (Series A to C) can be equipped with a multisensor module upon request. Only one chamber can be used when measuring simlutaneously fluoresecence and respiatin. The other chamber can not be used for any measuremts during fluorecsence experiment, but both chambers can still be used for standard repisration experiments (no fluoresecence). An O2k-MultiSensor electronic upgrading is NOT required for O2k (Series A to C). The modified O2k-Fluorescence Module will include fluorescence Sensors for only one chamber and only one black PEEK stoppers (with single central titration port). Additionally, a special pre-amplification box will be included.
