O2k-Fluo LED2-Module
| Description | The O2k-Fluorescence Module is a modular extension of the O2k-Core, adding a new dimension to HRR. Optical probes are inserted through the front window of the O2k-glass chambers, for measurement of hydrogen peroxide production (Amplex red), ATP production (Mg green), mt-membrane potential (Safranin), Ca2+ (Ca green), and numerous other applications open for O2k-user innovation. |
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| Product ID | 12100-01 |
| Type | O2k, O2k-Module, MultiSensor, Fluorescence Module"Fluorescence Module" is not in the list (O2k, O2k-Module, O2k-Core, O2k-Fluorometer, O2k-Main Unit, O2k-Assembly Kit, OroboPOS, OroboPOS-Service Kit, ISS, TIP2k, ...) of allowed values for the "Product type" property. |
| Link | @OROBOROS, Oxygraph-2k |
| Image |
MitoPedia methods:
Fluorometry
Components: O2k-Fluorescence Module
Please see also Upgrading the Oxygraph-2k Basic below!
The O2k-Fluorescence Module for O2k Series D and above consists of optical probes for both O2k-chambers (green and blue excitation), optical filters, front fixation of the optical probes to the glass windows of the O2k-chambers, electronic control of light intensity, data input into the O2k-Main Unit, and the new DatLab software.
- 2 Fluorescence sensors per chamber (4 in total), green and blue emission with photodiode and filters
- 1 Electronic Current Control for regulation of light intensity
- 1 Front Fixation for fluorescence sensors to O2k-glass chamber
- 1 Registration fee waived for the O2k-Fluorometry Workshop.
The O2k-Fluorescence Module for O2k Series A to C and above consists of optical probes for one O2k-chambers (green and blue excitation), optical filters, front fixation of the optical probes to the glass windows of the O2k-chambers, electronic control of light intensity, data input into the O2k-Main Unit, a preamplification box and the new DatLab software.
- 2 Fluorescence sensors for one chamber, green and blue emission with photodiode and filters
- 1 Electronic Current Control for regulation of light intensity
- 1 Front Fixation for fluorescence sensors to O2k-glass chamber
- 1 Registration fee waived for the O2k-Fluorometry Workshop.
- 1 Preamplification box.
References
- Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2011) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J. Comp. Physiol. B.[Epub ahead of print].
- Hunter FW, Wanga J, Patela R, Hsua H-L, Hickey AJ, Haya MP, Wilson WR (2012) Homologous recombination repair-dependent cytotoxicity of the benzotriazine di-N-oxide CEN-209: Comparison with other hypoxia-activated prodrugs. Biochem Pharmacol 83: 574–585.
- Fasching M, Harrison DK, Tretter L, Gnaiger E (2011) Combination of high-resolution respirometry and fluorometry for continuous monitoring of hydrogen peroxide production by mitochondria with resolution in the nanomolar range. Abstract Berlin.
- Sumbalova Z, Harrison DK, Gradl P, Fasching M, Gnaiger E (2011) Mitochondrial membrane potential, coupling control, H2O2 production, and the upper limit of mitochondrial performance. Abstract Kagoshima.
News
The first series of the O2k-Fluorescence Module will be completed by beginning of April 2012, and will be delivered in April 2012, for orders received by end of January 2012.
O2k-Fluorometry Workshop
- O2k-Fluorometry Workshop from 15 to 16 March 2012, Innsbruck, Austria.
Upgrading the Oxygraph-2k Basic
- The O2k-Core fully supports the O2k-Fluorescence Module.
- The Oxygraph-2k 20000-44 (Series D and E) requires the O2k-MultiSensor electronic upgrading. The O2k-MultiSensor System fully supports the O2k-Fluorescence Module. Two black PEEK stoppers (with single central titration port) are included in the O2k-Fluorescence Module.
- The Oxygraph-2k 20000-44 (Series A to C) can be equipped with a O2k-Fluorescence Module upon request. However, for best performance we recommend to use an O2k Series D and above. If Oxygraph-2k 20000-44 (Series A to C) is equipped with an O2k-Fluorescence Module only one chamber can be used when measuring simultaneously fluorescence and respiration. The other chamber can not be used for any measurements during fluorescence experiments, but both chambers can still be used for standard respiration experiments (no fluorescence). The O2k-MultiSensor electronic upgrading is NOT required to use the O2k-Fluorescence Module for O2k Series A to C. The modified O2k-Fluorescence Module will include fluorescence sensors for only one chamber (green and blue light source) and only one black PEEK stoppers (with single central titration port). Additionally, a special pre-amplification box will be included. See also the Discussion page.
