Difference between revisions of "O2k-Open Support alert"
From Bioblast
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*'''Jan-31 Fr''' | *'''Jan-31 Fr''' | ||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration''' | :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration''' | ||
::::::: In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). ''Further details in'' [[MitoPedia: DatLab |MitoPedia]]: Ā»[[Oxygen calibration - DatLab]] | ::::::: In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). ''Further details in'' [[MitoPedia: DatLab |MitoPedia]]: Ā» [[Oxygen calibration - DatLab]] Ā« | ||
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:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you check the O<sub>2</sub> flux at the end of the air calibration with the O2k-chamber closed?''' | :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you check the O<sub>2</sub> flux at the end of the air calibration with the O2k-chamber closed?''' | ||
::::::: In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O<sub>2</sub> slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. ''Further details in'' [[MitoPedia: DatLab |MitoPedia]]: [[Oxygen calibration - DatLab]] | ::::::: In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O<sub>2</sub> slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. ''Further details in'' [[MitoPedia: DatLab |MitoPedia]]: Ā» [[Oxygen calibration - DatLab]] Ā« | ||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you set mark āR1ā and complete the air calibration correctly?''' | :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you set mark āR1ā and complete the air calibration correctly?''' | ||
::::::: Your air calibration is not effective until you set mark āR1ā andclick on <span style="background:lightgrey">Calibrate and copy to clipboard</span style> in the <span style="background:lightgrey">O2 calibration</span style> window. ''Further details in'' [[MitoPedia: DatLab |MitoPedia]]: [[Oxygen calibration - DatLab]] Ā | ::::::: Your air calibration is not effective until you set mark āR1ā andclick on <span style="background:lightgrey">Calibrate and copy to clipboard</span style> in the <span style="background:lightgrey">O2 calibration</span style> window. ''Further details in'' [[MitoPedia: DatLab |MitoPedia]]: Ā» [[Oxygen calibration - DatLab]] Ā« | ||
*'''Jan-24 Fr''' | *'''Jan-24 Fr''' | ||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]]Ā [[File:Blue-info.png|40px|Info: improve]] '''Do you write the serial number of the polarographic oxygen sensor (POS) in the āO2k configurationā window?''' | :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]]Ā [[File:Blue-info.png|40px|Info: improve]] '''Do you write the serial number of the polarographic oxygen sensor (POS) in the āO2k configurationā window?''' | ||
::::::: Including the POS serial number in the Ā“O2k configurationĀ“ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed. | ::::::: Including the POS serial number in the Ā“O2k configurationĀ“ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed. Ā» [[O2k configuration]] Ā« | ||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you perform O2k-Chamber volume calibration?''' | :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you perform O2k-Chamber volume calibration?''' | ||
::::::: The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the [[O2k-sV-Module]]). | ::::::: The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the [[O2k-sV-Module]]). Ā» [[O2k-Chamber MitoPedia]] Ā« | ||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you use the correct O<sub>2</sub> solubility factor (F<sub>M</sub>) in the āO2 calibrationā window in DatLab? | :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you use the correct O<sub>2</sub> solubility factor (F<sub>M</sub>) in the āO2 calibrationā window in DatLab? | ||
::::::: The O<sub>2</sub> solubility factor varies according to the medium used and influences the air calibration. | ::::::: The O<sub>2</sub> solubility factor varies according to the medium used and influences the air calibration. Ā» [[Oxygen solubility factor]] Ā« |
Revision as of 10:50, 26 February 2020
O2k-Open Support alert |
We provide service and support based on open innovation
- - listed in the Oroboros Ecosystem Agenda 2020
Jan 2020
- Jan-31 Fr
- Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration
- In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). Further details in MitoPedia: Ā» Oxygen calibration - DatLab Ā«
- Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration
- Jan-30 Th
- Do you check the O2 flux at the end of the air calibration with the O2k-chamber closed?
- In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O2 slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. Further details in MitoPedia: Ā» Oxygen calibration - DatLab Ā«
- Do you check the O2 flux at the end of the air calibration with the O2k-chamber closed?
- Your air calibration is not effective until you set mark āR1ā andclick on Calibrate and copy to clipboard in the O2 calibration window. Further details in MitoPedia: Ā» Oxygen calibration - DatLab Ā«
- Jan-24 Fr
- Do you write the serial number of the polarographic oxygen sensor (POS) in the āO2k configurationā window?
- Including the POS serial number in the Ā“O2k configurationĀ“ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed. Ā» O2k configuration Ā«
- Do you write the serial number of the polarographic oxygen sensor (POS) in the āO2k configurationā window?
- Do you perform O2k-Chamber volume calibration?
- The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module). Ā» O2k-Chamber MitoPedia Ā«
- Do you perform O2k-Chamber volume calibration?
- Do you use the correct O2 solubility factor (FM) in the āO2 calibrationā window in DatLab?
- The O2 solubility factor varies according to the medium used and influences the air calibration. Ā» Oxygen solubility factor Ā«
- Do you use the correct O2 solubility factor (FM) in the āO2 calibrationā window in DatLab?