Difference between revisions of "O2k-chamber"

Revision as of 16:38, 11 May 2015

O2k-chamber

Description	O2k-Chamber: 16 mm inner diameter, Duran glass polished, with standard operation volume (V) of 2 cm³ (2 ml). Two units of this item are standard components of the O2k-Assembly Kit (O2k-Core). The optical properties of Duran allow application in the O2k-Fluorometer (Duran optical properties).
Product ID	23100-01
Type	O2k, O2k-Assembly Kit
Link	MiPNet09.01 O2k-ParadigmShift
Image

A spare (third) O2k-Chamber may be added to the items ordered with the O2k-System. For dispatch of the O2k, the O2k-Chambers are shipped in O2k-Service Box.

Cleaning the glass chamber

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Standard operating procedure - cleaning and storage of the O2k-Chamber: >> MiPNet19.03 O2k-cleaning and ISS

Chamber assembly

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

The assembly of the chamber is described in MiPNet19.18A_O2k-Start.

Possible problems

Chamber leaking liquids (medium is leaking out of the chamber)
Oxygen leak of the chamber (strongly negative intercept in the instrumental background test
Unstable O2 signal
Unexpected high or low O2 signal

Trouble shooting procedure

If the problem is obviously related to the chamber proceed to solutions. If the problem manifests in a very low or zero raw signal, the possible contribution of chamber assembly to the problem is easily checked by doing the first step of Locating_a_problem#Remove_components: if a very low or zero raw signal changes to the expected values (see Raw signal the problem is the chamber assembly.

Otherwise follow Locating a problem

If the problem is not located on the POS or the POS connector try solving the problem by reassembling the chamber before concluding that the main unit is defect.

Solutions

Disassemble the chamber by reversing the instructions in MiPNet19.18A O2k-Start.
Check for visible damages on the glass chamber, on the POS seal tip and all o-rings (on chamber holders or on stoppers). If a damage is visible replace the part or consult OROBOROS Instruments. If there is a deposit visible in the glass chamber see Cleaning the glass chamber.
Reassemble the chamber following exactly the description in MiPNet19.18A O2k-Start. Especially, use only small increments (not more than successive quarter turns of the POS holder) when lowering the chamber.

Chamber volume calibration

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

The volume calibration of the chamber is described in MiPNet19.18A_O2k-Start.

Old model stopper: if you use blue Titanium stoppers: check the manual delivered with your O2k or contact OROBOROS INSTRUMENTS (instruments@oroboros.at) for instructions.

Glass chamber stuck in the O2k

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Problem

After removing OroboPOS-Connector, OroboPOS-Holder, and O2k-Chamber Holder from the O2k , the O2k-Chamber cannot be removed. It remains stuck even after washing with water.

Solution

The reason for this problem is very likely medium that has been spilled and acts now as a glue between glass chamber and copper block. Wash with plenty of warm water and try to remove the chamber while the O2k is switched on and heated up to 37 °C. You can try GENTLY to use the sensor holder (not the sensor connector) to try to push up the glass chamber (like you do during chamber assembly) - while the O2k is warm and with plenty of warm water. Since the sensors are removed you also can use e.g. 40° (or a little bit more) for the washing / removing attempts.

@@ Line 2: / Line 2: @@
 |description='''O2k-Chamber''': 16 mm inner diameter, Duran glass polished, with standard operation volume (''V'') of 2 cm<sup>3</sup> (2 ml).
-Two units of this item are standard components of the [[O2k-Assembly Kit]] ([[O2k-Core]]).
+Two units of this item are standard components of the [[O2k-Assembly Kit]] ([[O2k-Core]]). The optical properties of Duran allow application in the [[O2k-Fluorometer]] ([http://www.duran-group.com/en/about-duran/duran-properties/optical-properties-of-duran.html  Duran optical properties]).
 |product id=23100-01
 |product type=O2k, O2k-Assembly Kit
-|info=[[MiPNet09.01 O2k-ParadigmShift]], [[Cleaning the glass chamber]], [[Chamber assembly]], [[Chamber volume calibration]]
+|info=[[MiPNet09.01 O2k-ParadigmShift]]
 |product image=[[Image:Glass chamber.jpg|right|180px]]
 }}
-* A spare (third) O2k-Chamber may be added to the items ordered with the O2k-System. For dispatch of the [[Oxygraph-2k]], the O2k-Chambers are shipped in [[O2k-Service Box]].
+A spare (third) O2k-Chamber may be added to the items ordered with the O2k-System. For dispatch of the [[Oxygraph-2k|O2k]], the O2k-Chambers are shipped in [[O2k-Service Box]].
-* For optical properties of Duran, see [http://www.duran-group.com/en/about-duran/duran-properties/optical-properties-of-duran.html  Duran optical properties]
-* [http://www.oroboros.at/?oxygraph2k-chamber Optimum chamber volume]
-* SOP: Cleaning and storage of the O2k-Chamber: >> [[MiPNet19.03 O2k-cleaning and ISS]].
-== O2k-Chamber technical support pages ==
+== Cleaning the glass chamber ==
 {{Technical support integrated}}
-=== Cleaning the glass chamber ===
+: Standard operating procedure - cleaning and storage of the O2k-Chamber: >> [[MiPNet19.03 O2k-cleaning and ISS]]
->> [[MiPNet19.03 O2k-cleaning and ISS]]
-=== Chamber assembly ===
+== Chamber assembly ==
-The assembly of the chamber and a procedure to position the glass chamber very accurately is described in [http://www.oroboros.at/index.php?id=o2k-start MiPNet12.06].
+{{Technical support integrated}}
-A wrongly positioned glass chamber can lead to a huge variety of problems, some obvious but others very difficult to understand.
-==== Possible Problems ====
-* chamber leaking liquids (medium is leaking out of the chamber)
-* oxygen leak of the chamber (strongly negative intercept in the [[instrumental background test]]
-* unstable O2 signal
-* unexpected high or low O2 signal
-==== Trouble shooting procedure ====
+: The assembly of the chamber is described in [[MiPNet19.18A_O2k-Start]].
-If the problem is obviously related to the chamber proceed to solutions. If the problem manifests in a very low or zero raw signal, the possible contribution of chamber assembly to the problem is easily checked by doing the first step of  [[Locating_a_problem#Remove_components]]: if a very low or zero raw signal changes to  the expected values (see [[Raw signal]] the problem is the chamber assembly.
-Otherwise follow  [[Locating a problem]]
+'''Possible problems'''
-If the problem is not located on the [[POS]] or the [[POS connector]] try solving the problem by reassembling the chamber before concluding that the [[main unit]] is defect.
+:* Chamber leaking liquids (medium is leaking out of the chamber)
+:* Oxygen leak of the chamber (strongly negative intercept in the [[instrumental background test]]
+:* Unstable O2 signal
+:* Unexpected high or low O2 signal
-==== Solutions ====
+'''Trouble shooting procedure'''
-* Disassemble the chamber by reversing the instructions in [[MiPNet19.18A O2k-Start]].
+: If the problem is obviously related to the chamber proceed to solutions. If the problem manifests in a very low or zero raw signal, the possible contribution of chamber assembly to the problem is easily checked by doing the first step of  [[Locating_a_problem#Remove_components]]: if a very low or zero raw signal changes to  the expected values (see [[Raw signal]] the problem is the chamber assembly.
-* Check for visible damages on the [[glass chamber]], on the [[POS seal tip]] and all o-rings (on chamber holders or on stoppers). If a damage is visible replace the part or consult OROBOROS Instruments. If there is a deposit visible in the glass chamber see [[Cleaning the glass chamber]].
-* Reassemble the chamber following exactly the description in [[MiPNet19.18A O2k-Start]]. Especially, use only small increments (not   more   than successive  quarter  turns of the POS holder) when lowering the chamber.
-=== Chamber volume calibration ===
+: Otherwise follow  [[Locating a problem]]
+: If the problem is not located on the [[POS]] or the [[POS connector]] try solving the problem by reassembling the chamber before concluding that the [[main unit]] is defect.
-For instructions how to carry out a [[Chamber O2k|chamber]] volume calibration you can choose from the following:
+'''Solutions'''
+:* Disassemble the chamber by reversing the instructions in [[MiPNet19.18A O2k-Start]].
+:* Check for visible damages on the [[glass chamber]], on the [[POS seal tip]] and all o-rings (on chamber holders or on stoppers). If a damage is visible replace the part or consult OROBOROS Instruments. If there is a deposit visible in the glass chamber see [[Cleaning the glass chamber]].
+:* Reassemble the chamber following exactly the description in [[MiPNet19.18A O2k-Start]]. Especially, use only small increments (not more  than successive quarter turns of the POS holder) when lowering the chamber.
-If you use white PVDF stoppers: [[MiPNet12.06]]
-If you use blue Titanium stoppers: check the manual delivered with your O2k or contact OROBOROS INSTRUMENTS ([mailto:instruments@oroboros.at instruments@oroboros.at]) to obtain a pdf file of the older manual version suitable for your stoppers.
+== Chamber volume calibration ==
-=== Glass chamber stuck in the O2k ===
+{{Technical support integrated}}
+: The volume calibration of the chamber is described in [[MiPNet19.18A_O2k-Start]].
+: Old model stopper: if you use blue Titanium stoppers: check the manual delivered with your O2k or contact OROBOROS INSTRUMENTS ([mailto:instruments@oroboros.at instruments@oroboros.at]) for instructions.
+== Glass chamber stuck in the O2k ==
+{{Technical support integrated}}
-==== Problem ====
+'''Problem'''
-After removing [[OroboPOS-Connector]], [[OroboPOS-Holder]], and [[O2k-Chamber Holder]]
+: After removing [[OroboPOS-Connector]], [[OroboPOS-Holder]], and [[O2k-Chamber Holder]] from the O2k , the [[O2k-Chamber]]  cannot be removed. It remains stuck even after washing with water.
-from the O2k , the [[O2k-Chamber]]  cannot be removed. It remains stuck even after washing with water.
-==== Solution ====
+'''Solution'''
-The reason for this problem is very likely medium that has been spilled and acts now as a glue between glass chamber and copper block.
+: The reason for this problem is very likely medium that has been spilled and acts now as a glue between glass chamber and copper block. Wash with plenty of warm water and try to remove the chamber while the O2k is switched on and heated up to 37 °C. You can try GENTLY to use the sensor holder (not the sensor connector) to try to push up the glass chamber (like you do during [[chamber assembly]]) - while the O2k is warm and with plenty of warm water.  Since the sensors are removed you also can use e.g. 40° (or a little bit more) for the washing / removing attempts.
-Wash with plenty of warm water and try to remove the chamber while the O2k is switched on and heated up to 37 °C. You can try GENTLY to use the sensor holder (not the sensor connector) to try to push up the glass chamber (like you do during [[chamber assembly]]) - while the O2k is warm and with plenty of warm water.  Since the sensors are removed you also can use e.g. 40° (or a little bit more) for the washing / removing attempts.