O2k-chamber

O2k-chamber

Description	O2k-Chamber: 16 mm inner diameter, Duran glass polished, with standard operation volume (V) of 2 cm³ (2 ml). Two units of this item are standard components of the O2k-Assembly Kit (O2k-Core).
Product ID	23100-01
Type	O2k, O2k-Assembly Kit
Link	MiPNet09.01 O2k-ParadigmShift, Cleaning the glass chamber, Chamber assembly, Chamber volume calibration
Image

A spare (third) O2k-Chamber may be added to the items ordered with the O2k-System. For dispatch of the Oxygraph-2k, the O2k-Chambers are shipped in O2k-Service Box.
For optical properties of Duran, see Duran optical properties
Optimum chamber volume
SOP: Cleaning and storage of the O2k-Chamber: >> MiPNet19.03 O2k-cleaning and ISS.

O2k-Chamber technical support pages

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Cleaning the glass chamber

>> MiPNet19.03 O2k-cleaning and ISS

Chamber assembly

The assembly of the chamber and a procedure to position the glass chamber very accurately is described in MiPNet12.06. A wrongly positioned glass chamber can lead to a huge variety of problems, some obvious but others very difficult to understand.

Possible Problems

chamber leaking liquids (medium is leaking out of the chamber)
oxygen leak of the chamber (strongly negative intercept in the instrumental background test
unstable O2 signal
unexpected high or low O2 signal

Trouble shooting procedure

If the problem is obviously related to the chamber proceed to solutions. If the problem manifests in a very low or zero raw signal, the possible contribution of chamber assembly to the problem is easily checked by doing the first step of Locating_a_problem#Remove_components: if a very low or zero raw signal changes to the expected values (see Raw signal the problem is the chamber assembly.

Otherwise follow Locating a problem If the problem is not located on the POS or the POS connector try solving the problem by reassembling the chamber before concluding that the main unit is defect.

Solutions

Disassemble the chamber by reversing the instructions in MiPNet19.18A O2k-Start.
Check for visible damages on the glass chamber, on the POS seal tip and all o-rings (on chamber holders or on stoppers). If a damage is visible replace the part or consult OROBOROS Instruments. If there is a deposit visible in the glass chamber see Cleaning the glass chamber.
Reassemble the chamber following exactly the description in MiPNet19.18A O2k-Start. Especially, use only small increments (not more than successive quarter turns of the POS holder) when lowering the chamber.

Chamber volume calibration

For instructions how to carry out a chamber volume calibration you can choose from the following:

If you use white PVDF stoppers: MiPNet12.06

If you use blue Titanium stoppers: check the manual delivered with your O2k or contact OROBOROS INSTRUMENTS (instruments@oroboros.at) to obtain a pdf file of the older manual version suitable for your stoppers.

Glass chamber stuck in the O2k

Problem

After removing OroboPOS-Connector, OroboPOS-Holder, and O2k-Chamber Holder from the O2k , the O2k-Chamber cannot be removed. It remains stuck even after washing with water.

Solution

The reason for this problem is very likely medium that has been spilled and acts now as a glue between glass chamber and copper block. Wash with plenty of warm water and try to remove the chamber while the O2k is switched on and heated up to 37 °C. You can try GENTLY to use the sensor holder (not the sensor connector) to try to push up the glass chamber (like you do during chamber assembly) - while the O2k is warm and with plenty of warm water. Since the sensors are removed you also can use e.g. 40° (or a little bit more) for the washing / removing attempts.