Difference between revisions of "OctPM pathway control state"
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{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=OctPM | ||
|description=''' | |description='''OctPM''': [[Octanoylcarnitine]] & [[Pyruvate]] & [[Malate]]. | ||
'''MitoPathway control:''' | '''MitoPathway control state:''' [[FN]] | ||
'''SUIT protocol:''' [[SUIT- | '''SUIT protocol:''' [[SUIT-002]], [[SUIT-005]] | ||
This substrate combination supports | This substrate combination supports N-linked flux which is typically higher than FAO capacity (F/FN<0 in the OXPHOS state). In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare [[malate-anaplerotic pathway control state]], M alone), and pyruvate is added to compare FAO as the background state with FN as the reference state. | ||
|info=[[MiPNet21.06 SUIT | |info=[[MiPNet21.06 SUIT RP]] | ||
}} | }} | ||
::: ''More details'' | |||
{{MitoPedia | ::::» [[FN]] | ||
|mitopedia | ::::» [[Additive effect of convergent electron flow]] | ||
::::» [[Respiratory_complexes#Respiratory_complexes_-_more_than_five |Respiratory complexes - more than five]] | |||
::::» [[OctPGM pathway control state]] | |||
::::» [[Convergent electron flow]] | |||
== OctPM<sub>''L''</sub> == | |||
FN pathway in the LEAK state can be evaluated in the following SUIT protocols: | |||
== OctPM<sub>''P''</sub> == | |||
FN pathway in the OXPHOS state can be evaluated in the following SUIT protocols: | |||
:::*[[SUIT-002]] | |||
::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-002 O2 mt D005]] | |||
::::* DL-Protocol for permeabilized fibers (pfi): [[SUIT-002 O2 pfi D006]] | |||
::::* DL-Protocol for permeabilized cells (pce): [[SUIT-002 O2 ce-pce D007]] | |||
:::*[[SUIT-005]] | |||
::::* DL-Protocol for permeabilized fibers (pfi):[[SUIT-005 O2 pfi D011]] | |||
== OctPM<sub>''E''</sub> == | |||
FN pathway in the ET state can be evaluated in the following SUIT protocols: | |||
---- | |||
[[File:SUIT-Reference-Protocol 1.jpg|700px |1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-]]<br /> | |||
---- | |||
[[File:SUIT-Reference-Protocol 2.jpg|700px |SUIT RP2]]<br /> | |||
---- | |||
{{MitoPedia concepts | |||
|mitopedia concept=SUIT state | |||
}} | }} | ||
Latest revision as of 04:59, 19 July 2022
- high-resolution terminology - matching measurements at high-resolution
OctPM pathway control state
Description
OctPM: Octanoylcarnitine & Pyruvate & Malate.
MitoPathway control state: FN
SUIT protocol: SUIT-002, SUIT-005
This substrate combination supports N-linked flux which is typically higher than FAO capacity (F/FN<0 in the OXPHOS state). In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare malate-anaplerotic pathway control state, M alone), and pyruvate is added to compare FAO as the background state with FN as the reference state.
Abbreviation: OctPM
Reference: MiPNet21.06 SUIT RP
OctPML
FN pathway in the LEAK state can be evaluated in the following SUIT protocols:
OctPMP
FN pathway in the OXPHOS state can be evaluated in the following SUIT protocols:
-
- DL-Protocol for isolated mitochondria and tissue homogenate (mt): SUIT-002 O2 mt D005
- DL-Protocol for permeabilized fibers (pfi): SUIT-002 O2 pfi D006
- DL-Protocol for permeabilized cells (pce): SUIT-002 O2 ce-pce D007
- DL-Protocol for permeabilized fibers (pfi):SUIT-005 O2 pfi D011
-
OctPME
FN pathway in the ET state can be evaluated in the following SUIT protocols:
MitoPedia concepts:
SUIT state
