Octanoylcarnitine

high-resolution terminology - matching measurements at high-resolution

Octanoylcarnitine

Description

Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine, frequently applied as a substrate for fatty acid oxidation (FAO) in mitochondrial preparations.

Abbreviation: Oct

Reference: pdf

MitoPedia topics: Substrate and metabolite 

» Fatty acid oxidation

» Carnitine O-octanoyltransferase

Application in HRR

Oct: Octanoylcarnitine ((±)-Octanoylcarnitine chloride, C₁₅H₃₀NO₄Cl); APExBIO Technology, Cat. No. B6371, 50 mg, store at -20°C; FW = 323.86.

Alternative: (±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.

TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at RT, desiccate); FW = 323.85.

General tips: For obtaining a higher solubility, warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.

Preparation of 100 mM stock solution (dissolved in H₂O or DMSO, can differ from batch to batch - check Certificate of Analysis):

1. Weigh 50 mg of octanoylcarnitine and dissolve in 1543 µL H₂O or DMSO.
2. Divide into 0.2 mL portions.
3. Store at -20 °C.

O2k manual titrations into 2 mL O2k-chamber

• Titration volume: 10 µL using a 25 µL syringe.
• Final concentration: 0.5 mM in MiR05 or MiR06. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05.