PGMS-pathway control state

high-resolution terminology - matching measurements at high-resolution

PGMS-pathway control state

Description

PGMS: Pyruvate & Glutamate & Malate & Succinate.

MitoPathway control state: NS-pathway control state

2-oxoglutarate is produced through the citric acid cycle from citrate by isocitrate dehydrogenase, from oxaloacetate and glutamate by the transaminase, and from glutamate by the glutamate dehydrogenase. If the 2-oxoglutarate carrier does not outcompete these sources of 2-oxoglutarate, then the TCA cycle operates in full circle with external pyruvate&malate&glutamate&succinate

Abbreviation: PGMS

Reference: Gnaiger 2014 MitoPathways - Chapter 5.6

MitoPedia concepts: SUIT state 

More details

» NS-pathway control state

» Additive effect of convergent electron flow

» Respiratory complexes - more than five

» Convergent electron flow

PGMS_L

PGMS pathway in the LEAK state can be evaluated in the following SUIT protocols:

PGMS_P

PGMS pathway in the OXPHOS state can be evaluated in the following SUIT protocols:

• SUIT-008

• DL-Protocol for permeabilized fibers (pfi): SUIT-008 O2 pfi D014
• DL-Protocol for permeabilized cells (pce): SUIT-008 O2 ce-pce D025

• SUIT-014

PGMS_E

PGMS pathway in the ET state can be evaluated in the following SUIT protocols:

• SUIT-001

• DL-Protocol for isolated mitochondria and tissue homogenate (mt): SUIT-001 O2 mt D001
• DL-Protocol for permeabilized fibers (pfi): SUIT-001 O2 pfi D002
• DL-Protocol for permeabilized cells (pce): SUIT-001 O2 ce-pce D003
• DL-Protocol for permeabilized PBMCs and PLTs: SUIT-001 O2 ce-pce D004

• SUIT-008

• DL-Protocol for permeabilized fibers (pfi): SUIT-008 O2 pfi D014
• DL-Protocol for permeabilized cells (pce): SUIT-008 O2 ce-pce D025

• SUIT-014

Discussion

Recent studies showed that S- and NS-linked OXPHOS capacity is inhibited by 2 mM malate concentrations as applied in many SUIT protocols. This inhibition is less pronounced at higher succinate concentrations (10 mM up to 50 mM S).