Difference between revisions of "Pecinova 2011 Mitochondrion"
| Line 7: | Line 7: | ||
|abstract=The primary attempt in diagnostic and experimental studies of numerous pathological states associated with mitochondrial dysfunction is a precise evaluation of changes in function, content and structure of mitochondrial OXPHOS system. The analysis of rat heart, liver, brain and kidney by oxygraphy, enzyme activities, membrane potential, and BN/SDS-PAGE western blotting demonstrated that tissue homogenates can substitute for isolated mitochondria, providing comparable qualitative mitochondrial parameters. The use of homogenate avoids the loss of the majority of mitochondria during their isolation. Only 50-100mg of the tissue is required for the complex OXPHOS analysis, i.e. five times less as compared with isolated mitochondria. | |abstract=The primary attempt in diagnostic and experimental studies of numerous pathological states associated with mitochondrial dysfunction is a precise evaluation of changes in function, content and structure of mitochondrial OXPHOS system. The analysis of rat heart, liver, brain and kidney by oxygraphy, enzyme activities, membrane potential, and BN/SDS-PAGE western blotting demonstrated that tissue homogenates can substitute for isolated mitochondria, providing comparable qualitative mitochondrial parameters. The use of homogenate avoids the loss of the majority of mitochondria during their isolation. Only 50-100mg of the tissue is required for the complex OXPHOS analysis, i.e. five times less as compared with isolated mitochondria. | ||
|keywords=Isolated mitochondria; Tissue homogenates; oxidative phosphorylation; Respiratory control; Mitochondrial membrane potential | |keywords=Isolated mitochondria; Tissue homogenates; oxidative phosphorylation; Respiratory control; Mitochondrial membrane potential | ||
|mipnetlab=CZ Prague Houstek J, CZ | |mipnetlab=CZ Prague Houstek J, CZ Hradec Kralove Cervinkova Z | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Rat | |organism=Rat | ||
|tissues=Heart, Nervous system, Liver, Kidney | |tissues=Heart, Nervous system, Liver, Kidney | ||
|preparations=Homogenate, Isolated Mitochondria | |preparations=Homogenate, Isolated Mitochondria | ||
|couplingstates=OXPHOS | |couplingstates=OXPHOS | ||
|instruments=Oxygraph-2k, TPP | |||
}} | }} | ||
Revision as of 16:27, 22 August 2014
| Pecinova A, Drahota Z, Nuskova H, Pecina P, Houstek J (2011) Evaluation of basic mitochondrial functions using rat tissue homogenates. Mitochondrion 11: 722-728. |
Pecinova A, Drahota Z, Nuskova H, Pecina P, Houstek J (2011) Mitochondrion
Abstract: The primary attempt in diagnostic and experimental studies of numerous pathological states associated with mitochondrial dysfunction is a precise evaluation of changes in function, content and structure of mitochondrial OXPHOS system. The analysis of rat heart, liver, brain and kidney by oxygraphy, enzyme activities, membrane potential, and BN/SDS-PAGE western blotting demonstrated that tissue homogenates can substitute for isolated mitochondria, providing comparable qualitative mitochondrial parameters. The use of homogenate avoids the loss of the majority of mitochondria during their isolation. Only 50-100mg of the tissue is required for the complex OXPHOS analysis, i.e. five times less as compared with isolated mitochondria. • Keywords: Isolated mitochondria; Tissue homogenates; oxidative phosphorylation; Respiratory control; Mitochondrial membrane potential
• O2k-Network Lab: CZ Prague Houstek J, CZ Hradec Kralove Cervinkova Z
Labels:
Organism: Rat
Tissue;cell: Heart, Nervous system, Liver, Kidney
Preparation: Homogenate, Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Coupling state: OXPHOS
HRR: Oxygraph-2k, TPP
