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Difference between revisions of "SUIT-001 O2 ce-pce D004"

From Bioblast
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{{MitoPedia
{{MitoPedia
|abbr=NSGp(PGM)
|abbr=NSGp(PGM)
|SUIT number=3
|SUIT number=1
|description=[[File:1PM;2D;2c;3U;4G;5S;6Rot;7Gp-.png|400px|SUIT-RP1 for PBMC and PLT]]
|description=[[File:1PM;2D;2c;3U;4G;5S;6Rot;7Gp-.png|400px|SUIT-RP1 for PBMC and PLT]]
|info='''A: SUIT 3 is the RP1 for PBMC and PLT''' - [[MiPNet21.06 SUIT RP]]
|info='''A: SUIT 1 (RP1) specific for PBMC and PTL cells''' - [[MiPNet21.06 SUIT RP]]
}}
}}
{{MitoPedia concepts
{{MitoPedia concepts
|mitopedia concept=SUIT protocol, SUIT A
|mitopedia concept=SUIT protocol, SUIT A
}}
}}
::: [[MitoPedia: SUIT]] - '''[[SUIT reference protocol]] RP1 for PBMC and PLT'''
::: [[MitoPedia: SUIT]] - '''[[SUIT reference protocol]] RP1 for PBMCs and PLTs'''
::: '''[[Categories of SUIT protocols|SUIT-category]]:''' NSGp(PGM)
::: '''[[Categories of SUIT protocols|SUIT-category]]:''' NSGp(PGM)
::: '''[[SUIT protocol pattern]]:''' orthogonal
::: '''[[SUIT protocol pattern]]:''' orthogonal




::::* Reference protocol 1 specifically for PBMC and PLT. Β 
::::* Reference protocol 1 specifically for PBMCs and PLTs. Β 
::::* The main difference between the RP1 for PBMC and PLT (SUIT3) and [[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-|SUIT RP1]] (SUIT1) is the absense of Oct in SUIT3. Β 
::::* The main difference between the RP1 for PBMCs and PLTs and RP1 for other mtprep [[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-|SUIT RP1]] is the absense of Oct in the case of blood cells. Β 


__TOC__
__TOC__
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== Harmonized SUIT protocols ==
== Harmonized SUIT protocols ==
'''SUIT RP1 for PBMC and PLT''' (SUIT 3) [[File:1PM;2D;2c;3U;4G;5S;6Rot;7Gp;8Ama;9AsTm;10Azd.png|400px|SUIT-RP1 for PBMC and PLT]] Β 
'''SUIT RP1 for PBMCs and PLTs''' [[File:1PM;2D;2c;3U;4G;5S;6Rot;7Gp;8Ama;9AsTm;10Azd.png|400px|SUIT-RP1 for PBMC and PLT]] Β 
'''SUIT RP2''' (SUIT 2) [[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.png|500px|SUIT-RP2]] Β 
'''SUIT RP2''' (SUIT 2) [[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.png|500px|SUIT-RP2]] Β 


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:::: [[1D;2M;3Oct;4M;5P;6G;7S;8Gp;9U;10Rot-|SUIT RP2]]:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;'''10Rot''';11Ama;'''12AsTm''';13Azd
:::: [[1D;2M;3Oct;4M;5P;6G;7S;8Gp;9U;10Rot-|SUIT RP2]]:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;'''10Rot''';11Ama;'''12AsTm''';13Azd


== SUIT O2 DLP for RP1 (DatLab 7.1) ==
== SUIT O2 DLP for RP1 (DatLab 7.3) ==


:::: [[DL-Protocols]] (DLP) for substrate-uncoupler-inhibitor titrations (see [[MitoPedia: SUIT]]) guide through a sequence of coupling and pathway control states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both. DL-Protocols for different SUIT protocols can be found in the library of protocols [[MitoPedia: SUIT]]. Instrumental DL-protocols are displayed in the [[DL-Protocols#DL-Protocol_library| DL-Protocol library]].
The DLP files for RP1 in PBMCs and PTLs can be found in the page: [[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-#SUIT_O2_DLP_for_SUIT_1_.28RP1.29_.28DatLab_7.3.29]]
Β 
{{Template:Download instructions}}
Β 
{| class="wikitable" border="1"
|-
! DL-Protocol
! Description
! Download - Protocol .DLP
! Download - Analysis .XLSX
|-
| SUIT 3 (RP1 for PBMC_PLT)
| SUIT reference protocol RP1 for blood samples
| style="text-align:center"| [[File:Download1.png|20px|link=http://wiki.oroboros.at/images/e/e4/RP1pce%28Dig%29_PBMC_PLT.DLP]]
| style="text-align:center"| [[File:Download2.png|20px|link=http://wiki.oroboros.at/images/e/ec/RP1pce%28Dig%29_blood.xlsx]]
|}





Revision as of 13:41, 13 June 2018


high-resolution terminology - matching measurements at high-resolution


SUIT-001 O2 ce-pce D004

Description

SUIT-RP1 for PBMC and PLT

Abbreviation: NSGp(PGM)

Reference: A: SUIT 1 (RP1) specific for PBMC and PTL cells - MiPNet21.06 SUIT RP

SUIT number: 1


MitoPedia concepts: SUIT protocol, SUIT A 

MitoPedia: SUIT - SUIT reference protocol RP1 for PBMCs and PLTs
SUIT-category: NSGp(PGM)
SUIT protocol pattern: orthogonal


  • Reference protocol 1 specifically for PBMCs and PLTs.
  • The main difference between the RP1 for PBMCs and PLTs and RP1 for other mtprep SUIT RP1 is the absense of Oct in the case of blood cells.


Harmonized SUIT protocols

SUIT RP1 for PBMCs and PLTs SUIT-RP1 for PBMC and PLT SUIT RP2 (SUIT 2) SUIT-RP2

SUIT RP1:1PM;2D;2c;3U;4G;5S;6Rot;7Gp;8Ama;9AsTm;10Azd
SUIT RP2:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd

SUIT O2 DLP for RP1 (DatLab 7.3)

The DLP files for RP1 in PBMCs and PTLs can be found in the page: 1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-#SUIT_O2_DLP_for_SUIT_1_.28RP1.29_.28DatLab_7.3.29


References