SUIT-002 O2 mt D005: Difference between revisions
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[[File:SUIT-Reference-Protocol.jpg|400px]] '''Harmonization between RP1 and RP2''' | [[File:SUIT-Reference-Protocol.jpg|400px]] '''Harmonization between RP1 and RP2''' | ||
== DL- | == DL-Protocols for SUIT protocol RP2 (DatLab 7.1)== | ||
{| class="wikitable" border="1" | [[DL-Protocols]] for substrate-uncoupler-inhibitor titrations (see [[MitoPedia: SUIT]]) proceed stepwise to activate a sequence of coupling control states and pathway control states. A specific SUIT protocol can be assigned to O2k-chamber A or B or both. | ||
{| class="wikitable" border="1" | |||
|- | |- | ||
! rowspan="2" style=text-align:center" | | ! rowspan="2" style=text-align:center" | DL-Protocol | ||
! rowspan="2" style=text-align:center" | Sample | ! rowspan="2" style=text-align:center" | Sample | ||
! colspan="2" style=text-align:center" | | ! colspan="2" style=text-align:center" | DLP File | ||
|- | |- | ||
| ''' | | style="text-align:center" | '''O<sub>2</sub>''' | ||
| | | style="text-align:center" |'''O<sub>2</sub>&Amp (H<sub>2</sub>O<sub>2</sub> production)''' | ||
| ''' | |||
|- | |- | ||
| RP2 | | RP2 | ||
| mt-preparations | | mt-preparations | ||
| ''In progress'' | | ''In progress'' | ||
| | | ''In progress'' | ||
|- | |- | ||
| RP2 | | RP2 | ||
| | | Permeabiliced cells (pce) with digitonin | ||
| [[Media: | | [[Media:RP1PCE(DIG).DLP|RP1pce(Dig)]] | ||
| ''In progress'' | |||
| | |||
|- | |- | ||
| RP2 | | RP2 | ||
| Permeabilized cells (pce) without digitonin | | Permeabilized cells (pce) without digitonin | ||
| | | ''In progress'' | ||
| ''In progress'' | |||
| | |||
|- | |- | ||
| RP2 | | RP2 | ||
| Permeabilized fibers | | Permeabilized fibers | ||
| | | ''In progress'' | ||
| ''In progress'' | |||
| | |- | ||
|} | |||
|} | |||
A full list of DL-Protocols (Instrumental DL-Protocols and SUIT DL-Protocols) is displayed in [[DL-Protocols]]. | |||
== DatLab-Analysis templates for | |||
== DatLab-Analysis templates for RP1 analysis (DatLab 7.1) == | |||
Line 83: | Line 77: | ||
! Sample | ! Sample | ||
! O2k-Core | ! O2k-Core | ||
|- | |- | ||
Line 89: | Line 82: | ||
| mt-preparations | | mt-preparations | ||
| [[Media:RP2mt.xlsx |»SUIT RP2mt]] | | [[Media:RP2mt.xlsx |»SUIT RP2mt]] | ||
|- | |- | ||
| | | RP2 | ||
| Permeabilized cells (pce) with digitonin | | Permeabilized cells (pce) with digitonin | ||
| [[Media:RP2pce(Dig).xlsx |»SUIT RP2pce(Dig)]] | | [[Media:RP2pce(Dig).xlsx |»SUIT RP2pce(Dig)]] | ||
|- | |- | ||
| | | RP2 | ||
| Permeabilized cells (pce) without digitonin | | Permeabilized cells (pce) without digitonin | ||
| [[Media:RP2pce.xlsx |»SUIT RP2pce]] | | [[Media:RP2pce.xlsx |»SUIT RP2pce]] | ||
|- | |- | ||
| | | RP2 | ||
| Permeabilized fibers | | Permeabilized fibers | ||
| [[Media:RP2mt.xlsx |»SUIT RP2mt]] | | [[Media:RP2mt.xlsx |»SUIT RP2mt]] |
Revision as of 10:43, 8 August 2017
Description
Abbreviation: FNSGp(PGM)
Reference: A: SUIT_RP2 - MiPNet21.06 SUIT RP
MitoPedia concepts:
SUIT protocol,
SUIT A
- MitoPedia: SUIT - SUIT reference protocol RP2
- SUIT-category: FNSGp(PGM)
- SUIT protocol pattern: diametral
Harmonized SUIT protocols
- SUIT RP1: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
- SUIT RP2: 1D;1M.1;2Oct;2c;2M2;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd
Harmonization between RP1 and RP2
DL-Protocols for SUIT protocol RP2 (DatLab 7.1)
DL-Protocols for substrate-uncoupler-inhibitor titrations (see MitoPedia: SUIT) proceed stepwise to activate a sequence of coupling control states and pathway control states. A specific SUIT protocol can be assigned to O2k-chamber A or B or both.
DL-Protocol | Sample | DLP File | |
---|---|---|---|
O2 | O2&Amp (H2O2 production)
| ||
RP2 | mt-preparations | In progress | In progress |
RP2 | Permeabiliced cells (pce) with digitonin | RP1pce(Dig) | In progress |
RP2 | Permeabilized cells (pce) without digitonin | In progress | In progress |
RP2 | Permeabilized fibers | In progress | In progress |
A full list of DL-Protocols (Instrumental DL-Protocols and SUIT DL-Protocols) is displayed in DL-Protocols.
DatLab-Analysis templates for RP1 analysis (DatLab 7.1)
SUIT Reference protocol | Sample | O2k-Core | |
---|---|---|---|
RP2 | mt-preparations | »SUIT RP2mt | |
RP2 | Permeabilized cells (pce) with digitonin | »SUIT RP2pce(Dig) | |
RP2 | Permeabilized cells (pce) without digitonin | »SUIT RP2pce | |
RP2 | Permeabilized fibers | »SUIT RP2mt | - |