SUIT-002 O2 mt D005: Difference between revisions

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:::: [[DL-Protocols]] (DLP) for substrate-uncoupler-inhibitor titrations (see [[MitoPedia: SUIT]]) guide through a sequence of coupling and pathway control states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both. Β 
:::: [[DL-Protocols]] (DLP) for substrate-uncoupler-inhibitor titrations (see [[MitoPedia: SUIT]]) guide through a sequence of coupling and pathway control states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both. Β 
{| class="wikitable" border="1"Β 
|-
! rowspan="2" style=text-align:center" | DL-Protocol
! rowspan="2" style=text-align:center" | Sample
! colspan="2" style=text-align:center" | DLP File
|-
| style="text-align:center" | '''O<sub>2</sub>'''
| style="text-align:center" |'''O<sub>2</sub>&Amp (H<sub>2</sub>O<sub>2</sub> production)'''
|-
| RP2
| mt-preparations
| ''In progress''
| ''In progress''
|-
| RP2
| Permeabiliced cells (pce) with digitonin
| ''In progress''Β 
| ''In progress''
|-
| RP2
| Permeabilized cells (pce) without digitonin
| ''In progress''
| ''In progress''
|-
| RP2
| Permeabilized fibers
| ''In progress''
| ''In progress''
|-
|}
A full list of DL-Protocols (Instrumental DL-Protocols and SUIT DL-Protocols) is displayedΒ  in [[DL-Protocols]].
== DatLab-Analysis templates for RP2 analysis (DatLab 7.1) ==




{| class="wikitable" border="1"
{| class="wikitable" border="1"
|-
|-
! SUIT Reference protocol
! DL-Protocol
! Sample
! Description
! O<sub>2</sub>
! Download - Protocol .DLP
Β 
! Download - Analysis .XLSX
|-
|-
| RP2
| RP2
| mt-preparations
| SUIT reference protocol RP2 for permeabilized cells (pce) with digitonin
| [[Media:RP2mt.xlsx |Β»SUIT RP2mt]]
| style="text-align:center"| [[File:Download1.png|20px|link=http://wiki.oroboros.at/images/3/32/RP2pce%28DIG%29.DLP]]
| style="text-align:center"| [[File:Download2.png|20px|link=http://wiki.oroboros.at/images/c/ca/RP2pce%28Dig%29.xlsx]]


|-
|-
| RP2
| RP2
| Permeabilized cells (pce) with digitonin
| SUIT reference protocol RP2 for mt-preparations
| [[Media:RP2pce(Dig).xlsx |Β»SUIT RP2pce(Dig)]]
| style="text-align:center"| [[File:Download1.png|20px|link=http://wiki.oroboros.at/images/5/53/RP2mt.DLP]]
| style="text-align:center"| [[File:Download2.png|20px|link=http://wiki.oroboros.at/images/9/9e/RP2mt.xlsx]]


|-
|-
| RP2
| RP2
| Permeabilized cells (pce) without digitonin
| SUIT reference protocol RP2 for permeabilized fibers
| [[Media:RP2pce.xlsx |Β»SUIT RP2pce]]
| style="text-align:center"| [[File:Download1.png|20px|link=http://wiki.oroboros.at/images/d/d1/RP2pfi.DLP]]
| style="text-align:center"| [[File:Download2.png|20px|link=http://wiki.oroboros.at/images/5/5b/RP2pfi.xlsx]]


|-
|-
| RP2
| RP2 blood
| Permeabilized fibers
| SUIT reference protocol RP2 for blood samples
| [[Media:RP2pfi.xlsx |Β»SUIT RP2mt]]
| style="text-align:center"| [[File:Download1.png|20px|link=http://wiki.oroboros.at/images/f/fe/RP2pce%28Dig%29_blood.DLP]]
| style="text-align:center"| [[File:Download2.png|20px|link=http://wiki.oroboros.at/images/0/09/RP2pce%28Dig%29_blood.xlsx]]
|}
|}



Revision as of 09:37, 28 August 2017


high-resolution terminology - matching measurements at high-resolution


SUIT-002 O2 mt D005

Description

1D;2OctM;3P;4G;5S;6Gp;7U;8Rot-.jpg

Abbreviation: FNSGp(PGM)

Reference: A: SUIT_RP2 - MiPNet21.06 SUIT RP


MitoPedia concepts: SUIT protocol, SUIT A 

MitoPedia: SUIT - SUIT reference protocol RP2
SUIT-category: FNSGp(PGM)
SUIT protocol pattern: diametral



Harmonized SUIT protocols

SUIT-RP1 SUIT RP1 SUIT-RP2 SUIT RP2

SUIT RP1: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
SUIT RP2: 1D;1M.1;2Oct;2c;2M2;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd



SUIT-Reference-Protocol.jpg Harmonization between RP1 and RP2


SUIT O2 DLP for RP2 (DatLab 7.1)

DL-Protocols (DLP) for substrate-uncoupler-inhibitor titrations (see MitoPedia: SUIT) guide through a sequence of coupling and pathway control states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both.


DL-Protocol Description Download - Protocol .DLP Download - Analysis .XLSX
RP2 SUIT reference protocol RP2 for permeabilized cells (pce) with digitonin Download1.png Download2.png
RP2 SUIT reference protocol RP2 for mt-preparations Download1.png Download2.png
RP2 SUIT reference protocol RP2 for permeabilized fibers Download1.png Download2.png
RP2 blood SUIT reference protocol RP2 for blood samples Download1.png Download2.png



References

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