SUIT-002 O2 mt D005: Difference between revisions
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:::: '''SUIT RP2''': 1D;1M.1;2Oct;2c;2M2;3P;4G;5S;6Gp;7U;'''8Rot''';9Ama;'''10Tm''';11Azd | :::: '''SUIT RP2''': 1D;1M.1;2Oct;2c;2M2;3P;4G;5S;6Gp;7U;'''8Rot''';9Ama;'''10Tm''';11Azd | ||
RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see [[Digitonin]]). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet [[Pathway control state|pathway control states]]. | |||
:::: [[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-|SUIT RP1 in cells]]: '''ce1''';1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;'''8Gp''';9Ama;'''10Tm''';11Azd | |||
:::: '''SUIT RP2 in cells''': '''ce1''';1Dig;1D;1M.1;2Oct;2c;2M2;3P;4G;5S;6Gp;7U;'''8Rot''';9Ama;'''10Tm''';11Azd | |||
Revision as of 09:49, 15 September 2017
Description
Abbreviation: FNSGp(PGM)
Reference: A: SUIT_RP2 - MiPNet21.06 SUIT RP
MitoPedia concepts:
SUIT protocol,
SUIT A
- MitoPedia: SUIT - SUIT reference protocol RP2
- SUIT-category: FNSGp(PGM)
- SUIT protocol pattern: diametral
Harmonized SUIT protocols
- SUIT RP1: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
- SUIT RP2: 1D;1M.1;2Oct;2c;2M2;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd
RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see Digitonin). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet pathway control states.
- SUIT RP1 in cells: ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
- SUIT RP2 in cells: ce1;1Dig;1D;1M.1;2Oct;2c;2M2;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd
Harmonization between RP1 and RP2
SUIT O2 DLP for RP2 (DatLab 7.1)
- DL-Protocols (DLP) for substrate-uncoupler-inhibitor titrations (see MitoPedia: SUIT) guide through a sequence of coupling and pathway control states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both. A list of DL-protocols is displayed in the DL-Protocol library.
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