Difference between revisions of "SUIT-003 O2 ce D009"
From Bioblast
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[[File:CCP_02-Rot,Ama.jpg|right|200px]] | [[File:CCP_02-Rot,Ama.jpg|right|200px]] | ||
== | == 1ce;2Omy;3U;4Rot;5Ama == | ||
:::* | :::* 1ce;2Omy;3U;4Rot;5Ama | ||
:::: In this CCP, ROX is measured after stepwise titration of [[rotenone]] (3Rot) and [[antimycin A]] (4Ama). In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (4Ama). | :::: In this CCP, ROX is measured after stepwise titration of [[rotenone]] (3Rot) and [[antimycin A]] (4Ama). In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (4Ama). | ||
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:::: Respiration in senescent human primary fibroblasts (0.2×10<sup>6</sup> cells/ml). Arrows show steps in the titration regime of the [[coupling control protocol]], inducing the following respiratory states: ROUTINE (''R''; routine state in cell-culture medium); LEAK (''L''; inhibition of ATPsynthase by 1 μg/ml oligomycin); ETS (''E''; maximal stimulation by uncoupling of oxidative phosphorylation in four subsequent titrations of the uncoupler, U, FCCP (2.5–4 μM final concentration); ROX (rotenone, Rot, and antimycin A; residual oxygen consumption). Modified after [[Huetter_2004_Biochem J]]. | :::: Respiration in senescent human primary fibroblasts (0.2×10<sup>6</sup> cells/ml). Arrows show steps in the titration regime of the [[coupling control protocol]], inducing the following respiratory states. | ||
::::* 1ce: ROUTINE (''R''; routine state in cell-culture medium); | |||
::::* 2Omy: LEAK (''L''; inhibition of ATPsynthase by 1 μg/ml oligomycin); | |||
::::* 3U: ETS (''E''; maximal stimulation by uncoupling of oxidative phosphorylation in four subsequent titrations of the uncoupler, U, FCCP (2.5–4 μM final concentration); | |||
::::* 4RotAma: ROX (rotenone, Rot, and antimycin A; residual oxygen consumption). Modified after [[Huetter_2004_Biochem J]]. | |||
:::* Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - [[Huetter_2004_Biochem J |»Bioblast link«]] | :::* Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - [[Huetter_2004_Biochem J |»Bioblast link«]] | ||
Revision as of 07:02, 26 June 2017
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 O2 ce D009
Description
Abbreviation: RLE
Reference: A Huetter 2004 Biochem J
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-category: RLE
- SUIT protocol pattern: linear coupling control protocol (CCP)
References
R;L;E format_ROX
- The CCP diagram may show the respiratory states directly (left) as an alternative to presenting the titration steps.
- Rot and Ama
- In this CCP, ROX is measured after titration of rotenone, antimycin A, or both in a single step. Validity of using rotenone only for inducing ROX in intact cells is evaluated by stepwise measurement of ROX after titration of rotenone (3Rot) followed by titration of animycin A (4Ama).
1ce;2Omy;3U;4Rot;5Ama
- 1ce;2Omy;3U;4Rot;5Ama
- In this CCP, ROX is measured after stepwise titration of rotenone (3Rot) and antimycin A (4Ama). In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (4Ama).
- Respiration in senescent human primary fibroblasts (0.2×106 cells/ml). Arrows show steps in the titration regime of the coupling control protocol, inducing the following respiratory states.
- 1ce: ROUTINE (R; routine state in cell-culture medium);
- 2Omy: LEAK (L; inhibition of ATPsynthase by 1 μg/ml oligomycin);
- 3U: ETS (E; maximal stimulation by uncoupling of oxidative phosphorylation in four subsequent titrations of the uncoupler, U, FCCP (2.5–4 μM final concentration);
- 4RotAma: ROX (rotenone, Rot, and antimycin A; residual oxygen consumption). Modified after Huetter_2004_Biochem J.
- Respiration in senescent human primary fibroblasts (0.2×106 cells/ml). Arrows show steps in the titration regime of the coupling control protocol, inducing the following respiratory states.
- Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - »Bioblast link«
