Difference between revisions of "SUIT-003 O2 ce D009"
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== References == | == References == | ||
{{#ask:[[Category:Publications]] [[Additional label::1ce; | {{#ask:[[Category:Publications]] [[Additional label::1ce;2ceOmy;3ceU-]] | ||
|?Was published in year=Year | |?Was published in year=Year | ||
|?Has title=Reference | |?Has title=Reference | ||
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:::* The CCP diagram may show the respiratory states directly (left) as an alternative to presenting the titration steps. | :::* The CCP diagram may show the respiratory states directly (left) as an alternative to presenting the titration steps. | ||
:::* '''Rot and Ama''' | :::* '''Rot and Ama''' | ||
:::: In this | :::: In this ceCCP, ROX is measured after titration of [[rotenone]], [[antimycin A]], or both in a single step. Validity of using [[rotenone]] only for inducing ROX in intact cells is evaluated by stepwise measurement of ROX after titration of rotenone (3Rot) followed by titration of animycin A (4ceAma). | ||
[[File:CCP_02-Rot,Ama.jpg|right|200px]] | [[File:CCP_02-Rot,Ama.jpg|right|200px]] | ||
== 1ce; | == 1ce;2ceOmy;3ceU;4ceRot;5ceAma == | ||
:::* 1ce; | :::* 1ce;2ceOmy;3ceU;4ceRot;5ceAma | ||
:::: In this | :::: In this ceCCP, ROX is measured after stepwise titration of [[rotenone]] (3ceRot) and [[antimycin A]] (4ceAma). In many intact cells ROX measured after titration of rotenone (3ceRot) is not or only slightly further inhibited by subsequent titration of animycin A (4ceAma). | ||
[[File:Huetter 2004 Biochem J.jpg|left|300px|CCP intact cells (fibroblasts)]] | [[File:Huetter 2004 Biochem J.jpg|left|300px|CCP intact cells (fibroblasts)]] | ||
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:::: Respiration in senescent human primary fibroblasts (0.2×10<sup>6</sup> cells/ml). Arrows show steps in the titration regime of the [[coupling control protocol]], inducing the following respiratory states. | :::: Respiration in senescent human primary fibroblasts (0.2×10<sup>6</sup> cells/ml). Arrows show steps in the titration regime of the [[coupling control protocol]], inducing the following respiratory states. | ||
::::* 1ce: ROUTINE (''R''; routine state in cell-culture medium); | ::::* 1ce: ROUTINE (''R''; routine state in cell-culture medium); | ||
::::* | ::::* 2ceOmy: LEAK (''L''; inhibition of ATPsynthase by 1 μg/ml oligomycin); | ||
::::* | ::::* 3ceU: ETS (''E''; maximal stimulation by uncoupling of oxidative phosphorylation in four subsequent titrations of the uncoupler, U, FCCP (2.5–4 μM final concentration); | ||
::::* | ::::* 4ceRotAma: ROX (rotenone, Rot, and antimycin A; residual oxygen consumption). Modified after [[Huetter_2004_Biochem J]]. | ||
:::* Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - [[Huetter_2004_Biochem J |»Bioblast link«]] | :::* Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - [[Huetter_2004_Biochem J |»Bioblast link«]] | ||
Revision as of 00:04, 5 August 2017
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 O2 ce D009
Description
Abbreviation: RLE
Reference: A Huetter 2004 Biochem J
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-category: RLE
- SUIT protocol pattern: linear coupling control protocol (CCP)
References
R;L;E format_ROX
- The CCP diagram may show the respiratory states directly (left) as an alternative to presenting the titration steps.
- Rot and Ama
- In this ceCCP, ROX is measured after titration of rotenone, antimycin A, or both in a single step. Validity of using rotenone only for inducing ROX in intact cells is evaluated by stepwise measurement of ROX after titration of rotenone (3Rot) followed by titration of animycin A (4ceAma).
1ce;2ceOmy;3ceU;4ceRot;5ceAma
- 1ce;2ceOmy;3ceU;4ceRot;5ceAma
- In this ceCCP, ROX is measured after stepwise titration of rotenone (3ceRot) and antimycin A (4ceAma). In many intact cells ROX measured after titration of rotenone (3ceRot) is not or only slightly further inhibited by subsequent titration of animycin A (4ceAma).
- Respiration in senescent human primary fibroblasts (0.2×106 cells/ml). Arrows show steps in the titration regime of the coupling control protocol, inducing the following respiratory states.
- 1ce: ROUTINE (R; routine state in cell-culture medium);
- 2ceOmy: LEAK (L; inhibition of ATPsynthase by 1 μg/ml oligomycin);
- 3ceU: ETS (E; maximal stimulation by uncoupling of oxidative phosphorylation in four subsequent titrations of the uncoupler, U, FCCP (2.5–4 μM final concentration);
- 4ceRotAma: ROX (rotenone, Rot, and antimycin A; residual oxygen consumption). Modified after Huetter_2004_Biochem J.
- Respiration in senescent human primary fibroblasts (0.2×106 cells/ml). Arrows show steps in the titration regime of the coupling control protocol, inducing the following respiratory states.
- Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - »Bioblast link«
