Difference between revisions of "SUIT-003 O2 ce D009"
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::::* 1ce: ROUTINE (''R''; routine state in cell-culture medium) in intact cells (ce); | ::::* 1ce: ROUTINE (''R''; routine state in cell-culture medium) in intact cells (ce); | ||
::::* 2ceOmy: LEAK (''L''; inhibition of ATPsynthase by 1 μg/ml oligomycin); | ::::* 2ceOmy: LEAK (''L''; inhibition of ATPsynthase by 1 μg/ml oligomycin); | ||
::::* 3ceU: | ::::* 3ceU: ET-pathway (''E''; maximal stimulation by uncoupling of oxidative phosphorylation in four subsequent titrations of the uncoupler, U, FCCP (2.5–4 μM final concentration); | ||
::::* 4ceRot: ROX (rotenone, Rot; residual oxygen consumption). | ::::* 4ceRot: ROX (rotenone, Rot; residual oxygen consumption). | ||
::::* 5ceAma: ROX (antimycin A, Ama; residual oxygen consumption). Modified after [[Huetter_2004_Biochem J]]. | ::::* 5ceAma: ROX (antimycin A, Ama; residual oxygen consumption). Modified after [[Huetter_2004_Biochem J]]. | ||
:::* Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - [[Huetter_2004_Biochem J |»Bioblast link«]] | :::* Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - [[Huetter_2004_Biochem J |»Bioblast link«]] | ||
Revision as of 14:05, 20 October 2017
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 O2 ce D009
Description
Abbreviation: ceRLE
Reference: A Huetter 2004 Biochem J
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-category: ceRLE
- SUIT protocol pattern: linear cell (ce) coupling control protocol (ceCCP)
References
R;L;E format_ROX
- The ceCCP diagram may show the respiratory states directly (left) as an alternative to presenting the titration steps.
- Rot and Ama
- In this ceCCP, ROX is measured after titration of rotenone, antimycin A, or both in a single step. Validity of using rotenone only for inducing ROX in intact cells is evaluated by stepwise measurement of ROX after titration of rotenone (3Rot) followed by titration of animycin A (4ceAma).
1ce;2ceOmy;3ceU;4ceRot;5ceAma
- 1ce;2ceOmy;3ceU;4ceRot;5ceAma
- In this ceCCP, ROX is measured after stepwise titration of rotenone (4ceRot) and antimycin A (5ceAma). In many intact cells ROX measured after titration of rotenone (4ceRot) is not or only slightly further inhibited by subsequent titration of animycin A (5ceAma).
- Respiration in senescent human primary fibroblasts (0.2×106 cells/ml). Arrows show steps in the titration regime of the coupling control protocol, inducing the following respiratory states.
- 1ce: ROUTINE (R; routine state in cell-culture medium) in intact cells (ce);
- 2ceOmy: LEAK (L; inhibition of ATPsynthase by 1 μg/ml oligomycin);
- 3ceU: ET-pathway (E; maximal stimulation by uncoupling of oxidative phosphorylation in four subsequent titrations of the uncoupler, U, FCCP (2.5–4 μM final concentration);
- 4ceRot: ROX (rotenone, Rot; residual oxygen consumption).
- 5ceAma: ROX (antimycin A, Ama; residual oxygen consumption). Modified after Huetter_2004_Biochem J.
- Respiration in senescent human primary fibroblasts (0.2×106 cells/ml). Arrows show steps in the titration regime of the coupling control protocol, inducing the following respiratory states.
- Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - »Bioblast link«
