Difference between revisions of "SUIT-003 O2 ce D009"
From Bioblast
| Line 4: | Line 4: | ||
|description=[[File:1ce;2ceOmy;3ceU-.jpg|150px]] | |description=[[File:1ce;2ceOmy;3ceU-.jpg|150px]] | ||
|SUIT number=D09_ce1;ce2Omy;ce3U;ce4Rot;ce5Ama | |SUIT number=D09_ce1;ce2Omy;ce3U;ce4Rot;ce5Ama | ||
|info='''A''' [[Huetter 2004 Biochem J]] | |info='''A''' [[Huetter 2004 Biochem J]] - [[SUIT-003]] | ||
}} | }} | ||
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ce | |||
| | |||
::: '''[[SUIT protocol pattern]]:''' linear cell (ce) [[coupling control protocol]] 1ce;2ceOmy;3ceU | ::: '''[[SUIT protocol pattern]]:''' linear cell (ce) [[coupling control protocol]] 1ce;2ceOmy;3ceU | ||
== References == | == References == | ||
| Line 50: | Line 46: | ||
:::* Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - [[Huetter_2004_Biochem J |»Bioblast link«]] | :::* Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - [[Huetter_2004_Biochem J |»Bioblast link«]] | ||
{{MitoPedia concepts | |||
|mitopedia concept=SUIT protocol, SUIT A | |||
}} | |||
{{MitoPedia methods | |||
|mitopedia method=Respirometry | |||
}} | |||
Revision as of 10:29, 15 January 2019
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 O2 ce D009
Description
Abbreviation: ce
Reference: A Huetter 2004 Biochem J - SUIT-003
SUIT number: D09_ce1;ce2Omy;ce3U;ce4Rot;ce5Ama
O2k-Application: O2
- SUIT-category: ce
- SUIT protocol pattern: linear cell (ce) coupling control protocol 1ce;2ceOmy;3ceU
References
R;L;E format_ROX
- The ceCCP diagram may show the respiratory states directly (left) as an alternative to presenting the titration steps.
- Rot and Ama
- In this ceCCP, ROX is measured after titration of rotenone, antimycin A, or both in a single step. Validity of using rotenone only for inducing ROX in intact cells is evaluated by stepwise measurement of ROX after titration of rotenone (3Rot) followed by titration of animycin A (4ceAma).
ce1;ce2Omy;ce3U;ce4Rot;ce5Ama
- ce1;ce2Omy;ce3U;ce4Rot;ce5Ama
- In this ceCCP, ROX is measured after stepwise titration of rotenone (ce4Rot) and antimycin A (ce5Ama). In many intact cells ROX measured after titration of rotenone (ce4Rot) is not or only slightly further inhibited by subsequent titration of animycin A (ce5Ama).
- Respiration in senescent human primary fibroblasts (0.2×106 cells/ml). Arrows show steps in the titration regime of the coupling control protocol, inducing the following respiratory states.
- 1ce: ROUTINE (R; routine state in cell-culture medium) in intact cells (ce);
- 2ceOmy: LEAK (L; inhibition of ATPsynthase by 1 μg/ml oligomycin);
- 3ceU: ET-pathway (E; maximal stimulation by uncoupling of oxidative phosphorylation in four subsequent titrations of the uncoupler, U, FCCP (2.5–4 μM final concentration);
- 4ceRot: ROX (rotenone, Rot; residual oxygen consumption).
- 5ceAma: ROX (antimycin A, Ama; residual oxygen consumption). Modified after Huetter_2004_Biochem J.
- Respiration in senescent human primary fibroblasts (0.2×106 cells/ml). Arrows show steps in the titration regime of the coupling control protocol, inducing the following respiratory states.
- Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - »Bioblast link«
MitoPedia concepts:
SUIT protocol,
SUIT A
MitoPedia methods:
Respirometry
