Difference between revisions of "SUIT-003 O2 ce D028"
From Bioblast
Capek Ondrej (talk | contribs) m (Reverted edits by Capek Ondrej (talk) to last revision by Doerrier Carolina) |
|||
Line 5: | Line 5: | ||
}} | }} | ||
{{MitoPedia concepts | {{MitoPedia concepts | ||
|mitopedia concept=SUIT protocol, SUIT | |mitopedia concept=SUIT protocol, SUIT B | ||
}} | }} | ||
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ceRLE(S) | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ceRLE(S) |
Revision as of 18:00, 10 November 2017
Description
Abbreviation: RLE(S)
Reference: A CCP(S)
MitoPedia concepts:
SUIT protocol,
SUIT B
- SUIT-category: ceRLE(S)
- SUIT protocol pattern: linear cell coupling control protocol (ceCCP)
ceR,L,E format
In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states.
- 4ceRot,5ceS;6ceAma
- 4ceRot: In many intact cells ROX measured after titration of rotenone (4ceRot) is not or only slightly further inhibited by subsequent titration of antimycin A.
- 5ceS: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria in dead cells (dce), respiration will be stimulated.
- 6ceAma: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).