Difference between revisions of "Safranin"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr=Saf | |abbr=Saf | ||
|description='''Safranin''' | |description='''Safranin''' is one of the most established dyes for measuring mitochondrial membrane potential by [[fluorometry]]. It is an [[extrinsic fluorophores |extrinsic fluorophore]] with an excitation wavelength of 495 nm and emission wavelength of 587 nm. | ||
|info=[[ | |info=[[Kauppinen 1984 Am J Physiol]], [[Krumschnabel 2014 Methods Enzymol]] | ||
}} | }} | ||
{{MitoPedia methods | {{MitoPedia methods | ||
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== Application in high-resolution respirometry == | == Application in high-resolution respirometry == | ||
Safranin is a potent inhibitor of Complex I linked respiration and of the phosphorylation system. At a concentration of 2 µM, safranin can be applied for simultaneous monitoring of oxygen consumption and mtMP of mt-preparations in a CII linked substrate state.<ref> Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-181. [[Krumschnabel 2014 Methods Enzymol |»Pubmed | Safranin is a potent inhibitor of Complex I-linked respiration and of the phosphorylation system. At a concentration of 2 µM, safranin can be applied for simultaneous monitoring of oxygen consumption and mtMP of mt-preparations in a CII-linked substrate state.<ref> Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-181. [[Krumschnabel 2014 Methods Enzymol |»Pubmed 24862266«]]</ref> | ||
The OROBOROS MitoLab uses Safranin O from Sigma (#S2255, 25 g). | |||
::» More details: [[Talk:Safranin |»Discussion«]]. | |||
::» Protocol on data acquisition and analysis with O2k-Fluorometry [[MiPNet19.19__Safranin_Data_Acquisition_and_Analysis| »MiPNet19.19«]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
* [[Figueira 2012 Methods Mol Biol]] | |||
* [[Komary 2010 Biochim Biophys Acta]] | |||
* [[Kiss 2014 FASEB J]] | |||
* [[Pham 2014 Am J Physiol]] | |||
Revision as of 12:20, 6 June 2015
- high-resolution terminology - matching measurements at high-resolution
Safranin
Description
Safranin is one of the most established dyes for measuring mitochondrial membrane potential by fluorometry. It is an extrinsic fluorophore with an excitation wavelength of 495 nm and emission wavelength of 587 nm.
Abbreviation: Saf
Reference: Kauppinen 1984 Am J Physiol, Krumschnabel 2014 Methods Enzymol
MitoPedia methods:
Fluorometry
Synonyms
Safranin O, Safranin Y, Saranin T, Gossypimine, Cotton Red, Basic Red2
Application in high-resolution respirometry
Safranin is a potent inhibitor of Complex I-linked respiration and of the phosphorylation system. At a concentration of 2 µM, safranin can be applied for simultaneous monitoring of oxygen consumption and mtMP of mt-preparations in a CII-linked substrate state.[1]
The OROBOROS MitoLab uses Safranin O from Sigma (#S2255, 25 g).
- » More details: »Discussion«.
- » Protocol on data acquisition and analysis with O2k-Fluorometry »MiPNet19.19«
References
- ↑ Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-181. »Pubmed 24862266«
