Difference between revisions of "Safranin"
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== Application in high-resolution respirometry == | == Application in high-resolution respirometry == | ||
Safranin is a potent inhibitor of Complex I-linked respiration and of the phosphorylation system. At a concentration of 2 µM, safranin can be applied for simultaneous monitoring of oxygen consumption and mtMP of mt-preparations in a CII-linked substrate state.<ref> Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163- | Safranin is a potent inhibitor of Complex I-linked respiration and of the phosphorylation system. At a concentration of 2 µM, safranin can be applied for simultaneous monitoring of oxygen consumption and mtMP of mt-preparations in a CII-linked substrate state.<ref> Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. [[Krumschnabel 2014 Methods Enzymol |»Bioblast link«]]</ref> | ||
:» '''O2k-Protocol''': O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. [[MiPNet20.13 Safranin mt-membranepotential| »MiPNet20.13«]] | :» '''O2k-Protocol''': O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. [[MiPNet20.13 Safranin mt-membranepotential| »MiPNet20.13«]] | ||
Revision as of 13:37, 9 June 2015
- high-resolution terminology - matching measurements at high-resolution
Safranin
Description
Safranin is one of the most established dyes for measuring mitochondrial membrane potential by fluorometry. It is an extrinsic fluorophore with an excitation wavelength of 495 nm and emission wavelength of 587 nm.
Abbreviation: Saf
Reference: Kauppinen 1984 Am J Physiol, Krumschnabel 2014 Methods Enzymol
MitoPedia methods:
Fluorometry
Synonyms
Safranin O, Safranin Y, Saranin T, Gossypimine, Cotton Red, Basic Red2
Application in high-resolution respirometry
Safranin is a potent inhibitor of Complex I-linked respiration and of the phosphorylation system. At a concentration of 2 µM, safranin can be applied for simultaneous monitoring of oxygen consumption and mtMP of mt-preparations in a CII-linked substrate state.[1]
- » O2k-Protocol: O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. »MiPNet20.13«
References
- ↑ Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. »Bioblast link«
