Difference between revisions of "Substrate-uncoupler-inhibitor titration"
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{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Respirometry | |mitopedia method=SUIT protocol, Respirometry | ||
|type=Respiration | |type=Respiration | ||
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{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Respiratory state | |mitopedia topic=Respiratory state, Inhibitor, Substrate and metabolite, Uncoupler | ||
|type=Respiration | |type=Respiration | ||
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Revision as of 09:22, 24 January 2016
- high-resolution terminology - matching measurements at high-resolution
Substrate-uncoupler-inhibitor titration
Description
Mitochondrial Substrate-uncoupler-inhibitor titration (SUIT) protocols are used with mt-preparations to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations within a single experimental assay.
Abbreviation: SUIT
Reference: Gnaiger 2014 MitoPathways, MiPNet09.12
MitoPedia methods: "SUIT protocol" is not in the list (Respirometry, Fluorometry, Spectrophotometry) of allowed values for the "MitoPedia method" property.
SUIT protocol"SUIT protocol" is not in the list (Respirometry, Fluorometry, Spectrophotometry) of allowed values for the "MitoPedia method" property.,
Respirometry
MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.,
Inhibitor,
Substrate and metabolite,
Uncoupler
SUIT protocols
SUIT protocols have been developed for the first time on the basis of high-resolution respirometry, since their application depends critically on long-term stability of the polarographic oxygen sensor (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: Gnaiger_2000_MitoInTheCold; MiR06) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature.
Attempts are underway to develop a database of SUIT protocols: the SUIT protocol library.
