Difference between revisions of "Substrate-uncoupler-inhibitor titration"
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|type=Respiration | |type=Respiration | ||
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Contributed by [[Gnaiger E]] 2010-09-18, edited 2016-01-24. | Contributed by [[Gnaiger E]] 2010-09-18, edited 2016-01-24. | ||
[[File:Pesta_2012_Methods_Mol_Biol_Fig7A.jpg|right|500px|link=Pesta_2012_Methods_Mol_Biol|Pesta and Gnaiger 2012]] | [[File:Pesta_2012_Methods_Mol_Biol_Fig7A.jpg|right|500px|link=Pesta_2012_Methods_Mol_Biol|Pesta and Gnaiger 2012]] | ||
==SUIT protocols== | ==SUIT protocols== | ||
SUIT protocols have been developed for the first time on the basis of [[high-resolution respirometry]], since their application depends critically on long-term stability of the [[polarographic oxygen sensor]] (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: [[Gnaiger_2000_MitoInTheCold]]; [[MiR06]]) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature. | :::: SUIT protocols have been developed for the first time on the basis of [[high-resolution respirometry]], since their application depends critically on long-term stability of the [[polarographic oxygen sensor]] (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: [[Gnaiger_2000_MitoInTheCold]]; [[MiR06]]) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature. | ||
Attempts are underway to develop a database of SUIT protocols: the [[SUIT protocol library]]. | :::: Attempts are underway to develop a database of SUIT protocols: the [[SUIT protocol library]]. | ||
==References== | ==References== | ||
* [[Boushel 2007 Diabetologia]] | ::::* [[Boushel 2007 Diabetologia]] | ||
* [[Gnaiger 2007 MitoPathways]] » ''new edition:'' [[Gnaiger 2014 MitoPathways]] | ::::* [[Gnaiger 2007 MitoPathways]] » ''new edition:'' [[Gnaiger 2014 MitoPathways]] | ||
* [[Gnaiger 2009 Int J Biochem Cell Biol]] | ::::* [[Gnaiger 2009 Int J Biochem Cell Biol]] | ||
* [[Lemieux 2011 Int J Biochem Cell Biol]] | ::::* [[Lemieux 2011 Int J Biochem Cell Biol]] | ||
* [[Pesta 2012 Methods Mol Biol]] | ::::* [[Pesta 2012 Methods Mol Biol]] | ||
* [[Pesta 2011 Am J Physiol Regul Integr Comp Physiol]] | ::::* [[Pesta 2011 Am J Physiol Regul Integr Comp Physiol]] | ||
* [[Sjoevall 2010 Crit Care]] | ::::* [[Sjoevall 2010 Crit Care]] | ||
Revision as of 22:37, 17 February 2016
- high-resolution terminology - matching measurements at high-resolution
Substrate-uncoupler-inhibitor titration
Description
Mitochondrial Substrate-uncoupler-inhibitor titration (SUIT) protocols are used with mt-preparations to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations within a single experimental assay.
Abbreviation: SUIT
Reference: Gnaiger 2014 MitoPathways, MiPNet09.12
MitoPedia concepts: "MitoFit Quality Control System" is not in the list (MiP concept, Respiratory state, Respiratory control ratio, SUIT concept, SUIT protocol, SUIT A, SUIT B, SUIT C, SUIT state, Recommended, ...) of allowed values for the "MitoPedia concept" property.
MitoFit Quality Control System"MitoFit Quality Control System" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
MitoPedia methods:
Respirometry
MitoPedia O2k and high-resolution respirometry: "SUIT protocol" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property.
SUIT protocol"SUIT protocol" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property.
MitoPedia topics:
Inhibitor,
Substrate and metabolite,
Uncoupler
Contributed by Gnaiger E 2010-09-18, edited 2016-01-24.
SUIT protocols
- SUIT protocols have been developed for the first time on the basis of high-resolution respirometry, since their application depends critically on long-term stability of the polarographic oxygen sensor (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: Gnaiger_2000_MitoInTheCold; MiR06) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature.
- Attempts are underway to develop a database of SUIT protocols: the SUIT protocol library.
