Difference between revisions of "Succinate"
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{{MitoPedia | {{MitoPedia | ||
|abbr=S | |abbr=S | ||
|description=''' | |description=[[File:Succinic_acid.jpg|left|100px|Succinic acid]] | ||
'''Succinic acid''', C<sub>4</sub>H<sub>6</sub>O<sub>4</sub>, occurs under physiological conditions as the anion '''succinate<sup>2-</sup>, M''', with ''p''K<sub>a1</sub> = 4.2 and ''p''K<sub>a2</sub> = 5.6. | |||
Succinate is formed in the [[TCA cycle]], and is a substrate of [[CII]], reacting to [[fumarate]] and feeding electrons into the [[Q-junction]]. Succinate (CII-linked) and NADH (CI-linked) provide convergent electron entries into the Q-junction. Succinate is transported across the inner mitochondrial membrane by the [[dicarboxylate carrier]]. | |||
|info=[http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways] | |info=[http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways] | ||
|type=Respiration | |type=Respiration | ||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''S: Succinate''' (Succinate disodium salt, hexahydrate, C<sub>4</sub>H<sub>4</sub>0<sub>4</sub>Na<sub>2</sub> * (H<sub>2</sub>O)<sub>6</sub>); Sigma S 2378, 100 g, store at | '''S: Succinate''' (Succinate disodium salt, hexahydrate, C<sub>4</sub>H<sub>4</sub>0<sub>4</sub>Na<sub>2</sub> * (H<sub>2</sub>O)<sub>6</sub>); Sigma S 2378, 100 g, store at RT; FW = 270.1 | ||
'''Preparation of 1 M stock solution''' (dissolved in H<sub>2</sub>O): | '''Preparation of 1 M stock solution''' (dissolved in H<sub>2</sub>O): | ||
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'''Oxygraph-2k | '''Oxygraph-2k manual titrations:''' [[MiPNet09.12 O2k-Titrations]] | ||
::* In the absence of CI-linked substrates, add the CI-inhibitor [[rotenone]] before addition of succinate, to avoid accumulation of [[oxaloacetate]] with subsequent inhibition of succiante dehydrogenase. | ::* In the absence of CI-linked substrates, add the CI-inhibitor [[rotenone]] before addition of succinate, to avoid accumulation of [[oxaloacetate]] with subsequent inhibition of succiante dehydrogenase. | ||
Revision as of 08:28, 15 April 2014
- high-resolution terminology - matching measurements at high-resolution
Succinate
Description
Succinic acid, C4H6O4, occurs under physiological conditions as the anion succinate2-, M, with pKa1 = 4.2 and pKa2 = 5.6. Succinate is formed in the TCA cycle, and is a substrate of CII, reacting to fumarate and feeding electrons into the Q-junction. Succinate (CII-linked) and NADH (CI-linked) provide convergent electron entries into the Q-junction. Succinate is transported across the inner mitochondrial membrane by the dicarboxylate carrier.
Abbreviation: S
Reference: Gnaiger 2012 MitoPathways
MitoPedia topics: Substrate and metabolite
Application in HRR
S: Succinate (Succinate disodium salt, hexahydrate, C4H404Na2 * (H2O)6); Sigma S 2378, 100 g, store at RT; FW = 270.1
Preparation of 1 M stock solution (dissolved in H2O):
- 1) Weigh 1.3505 g of succinate and dissolve in 3 ml H2O.
- 2) Check pH and adjust to 7.0 if necessary with 1 N HCl (usually the pH is 7 without any adjustment).
- 3) Transfer to 5 ml volumetric glass flask and adjust the final volume to 5 ml.
- 4) Divide into 0.5 ml portions.
- 5) Store frozen at -20 °C.
Oxygraph-2k manual titrations: MiPNet09.12 O2k-Titrations
- In the absence of CI-linked substrates, add the CI-inhibitor rotenone before addition of succinate, to avoid accumulation of oxaloacetate with subsequent inhibition of succiante dehydrogenase.
- When keeping the succinate stock solution on ice, check for complete solubilization of succinate and warm in your hands if necessary.
- Titration volume: 20 µl using a 50 µl syringe (2 ml O2k-chamber).
- Final concentration: 10 mM.
