Talk:NextGen-O2k Scientific developments: Difference between revisions
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== Track record == | == Track record == | ||
:::: '''2021-06-09:'' | :::: '''2021-06-09:''' "Coenzyme Q redox state and high-resolution respirometry: Novel perspectives for mitochondrial diagnostics" was presented at [[ESCI 2021 Virtual]]. | ||
:::: '''2021-05-20:''' Oxygen dependence of photosynthesis and light-enhanced dark respiration | :::: '''2021-05-20:''' The poster "Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry" was presented at [[ISAP 2021 Virtual]]. | ||
:::: '''2021-04-30:''' MitoFit preprint on the PB-Module: [[Went 2021 MitoFit PB|Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry]] | :::: '''2021-04-30:''' MitoFit preprint on the PB-Module: [[Went 2021 MitoFit PB|Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry]] | ||
:::: '''2021-02-18:''' MitoFit preprint publication: [[Komlodi 2021 MitoFit Q|Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria]] | :::: '''2021-02-18:''' MitoFit preprint publication: [[Komlodi 2021 MitoFit Q|Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria]] |
Revision as of 12:59, 21 June 2021
Track record
- 2021-06-09: "Coenzyme Q redox state and high-resolution respirometry: Novel perspectives for mitochondrial diagnostics" was presented at ESCI 2021 Virtual.
- 2021-05-20: The poster "Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry" was presented at ISAP 2021 Virtual.
- 2021-04-30: MitoFit preprint on the PB-Module: Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry
- 2021-02-18: MitoFit preprint publication: Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria
- 2021-02-09: Start of the last biological replicates measuring oxygen kinetics of the contribution to total dark respiration of the AOX-Q branch in Chlamydomonas reinhardtii.
- 2021-01-29: Deliverable 2.1 submitted.
- 2021-01-29: MitoFit preprint publication: Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration
- 2020-12-04: Start of the development of a white PB-Sensor for the PB-Module in collaboration with the algae KOLs
- 2020-11-30: The poster showing the latest scientific developments on the PB-Module, presented on the AlgaEurope 2020, was published in MitoFit Preprints: High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii β towards biotechnology exploitations
- 2020-11-06: SUIT protocols (SUIT-030 family) to determine the maximal photosynthesis rate under light- and inorganic carbon-saturating conditions optimized for microalgal living cells.
- 2020-10-27: First tests of the NADH-Module integrated in the NextGen-O2k.
- 2020-10-22: Optimization of the optimal conditions for measuring photosynthesis in the marine microalgae Phaeodactylum tricornutum with the PB-Module.
- 2020-10-08: Laurent Beck started with measurements on Chlamydomonas reinhardtii using the PB- and Q-Modules , supervised by PI Maria Huete-Ortega and Thomas Roach (University of Innsbruck, Institute of Botany)
- 2020-07-23: First isolation of mitochondria in microalgae trials
- 2020-07-01: Start of the development of protocols for permeabilizing microalgae cells
- 2020-04-24: First tests with pH electrodes
- 2020-03-11: First tests with Calcium Green
- 2020-02-20: Started development of protocols (and DL-Protocols) for the PB-Module, including new SUIT protocols for photosynthesis measurements.
- 2019-12-31: Determination of optimal Q-sensor measurement conditions for different biological samples (brain and heart isolated mitochondria from mouse, yeast)
- 2019-12-06: First version of the Cyclic Voltammetry Manual released: CV-Module
- 2019-12-02: Optimization of O2k-Procedures for isolated mitochondria preparations
- 2019-11-22: First version of NextGen-O2k Manual/SOP finalized: NextGen-O2k: Q-Module
- 2019-11-21: Updated beta version of cyclic voltammetry provided to the Key Opinion Leaders for testing
- 2019-11-18: Application of Q-redox state measurements in scientific projects
- 2019-10-22: 66 SUIT protocols for different O2k-Applications (i.e. oxygen consumption, hydrogen peroxide production, mitochondrial membrane potential, ATP production) which can be measured in living cells, permeabilized cells, isolated mitochondria, tissue homogenate and permeabilized fibers optimized and released with DatLab 7.4, see MitoPedia:_SUIT
- 2019-10-21: Four new videos for DatLab 7.4 software released - see: O2k-Videosupport DatLab 7.4
- 2019-09-24: Two new videos for mitochondrial preparations released - see: O2k-Videosupport Mitochondrial preparations
- 2019-09-23: First beta version of the cyclic voltammetry used by the Key Opinion Leaders during 1st Q-Module workshop at the Oroboros O2k-Workshop IOC141
- 2019-09-20: First six videos for the Q-Module released - see: O2k-Videosupport Q-Module
- 2019-09-06: Development of two harmonized SUIT protocols (and DL-Protocols) for the Q-Module, both provided to the Key Opinion Leaders
- 2019-07-17: First tests of the new O2 analysis template DL7.4
- 2019-07: Started development of SUIT protocols for isolated mitochondria and permeabilzied cells
- 2019-06-18: Testing of the SUITbrowser by the participants at the Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC139
- 2019-06: Integration of cyclic voltammetry as a quality control step into the Q-Module
- 2019-06: Testing of the SUITbrowser