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Cardoso 2021 MitoFit MgG

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Cardoso 2021 MitoFit MgG

Publications in the MiPMap
Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. https://doi.org/10.26124/mitofit:2021-00012021-06-30 published in Bioenerg Commun 2021.1.

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Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration


MitoFit Preprints (2021-01-29) MitoFit Prep

Abstract: Journal publication 2021-06-30 in »Bioenergetics Communications 2021.1«

For the advanced study of mitochondrial function, high-resolution respirometry is extended by fluorometric measurement of ATP production using the fluorophore Magnesium Green™ (MgG). A common problem with several fluorescent dyes is the inhibition of mitochondrial respiration. In the present study, a coupling control protocol was applied in combination with MgG to measure ATP production simultaneously with respiration for calculation of P»/O2 ratios. MgG at 1.1 µM did not affect respiration through the NADH-linked and succinate-linked pathways. Respiration was not inhibited in any of the coupling control states, hence coupling control efficiencies were not affected by MgG. Keywords: ATP, ATP production, high-resolution respirometry, Magnesium Green, mitochondria, oxidative phosphorylation, fluorometry, FluoRespirometry Bioblast editor: Gnaiger E

ORCID: ORCID.png Cardoso Luiza HD, ORCID.png Doerrier Carolina, ORCID.png Gnaiger Erich

Data availability

Original files are available Open Access at Zenodo repository: 10.5281/zenodo.4032674

Keywords


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Bioblast links: ATP production - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>



Phosphorylation pathway

» Phosphorylation pathway
» ATP synthase
» Adenine nucleotide translocase
» Phosphate carrier
Phosphorylation pathway substrates
» ADP
» ATP
» Inorganic phosphate
Phosphorylation pathway inhibitors
» Oligomycin
» Carboxyatractyloside
» Atractyloside
» Bongkrekik acid

Coupling control

» Oxidative phosphorylation
» OXPHOS capacity
» P-L control efficiency
» ROUTINE respiration
» R-L control efficiency

Respiratory complexes and coupling

» Complex I
» Complex III
» Complex IV
» Proton pump
» Electron-transfer-pathway state

ATP production measurement

» Magnesium Green


References (Open Access versus Paywall)

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Supported by project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 859770.