Talk:O2k-Open Support alert: Difference between revisions

Revision as of 14:47, 4 March 2020

Do you perform the leaky chamber test after chamber assembly?

After chamber reassembly you might experience a small leaky chamber only identified by a leaky chamber test- »Leaky chamber test«

Do you change the slope smoothing to 20 while running experiments with biological sample?

While running your experiment, especially during uncoupler titrations, it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - »Slope smoothing«

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 :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you perform the ''leaky chamber test'' after chamber assembly? '''
 ::::::: After chamber reassembly you might experience a small leaky chamber only identified by a ''leaky chamber test''- »[https://www.bioblast.at/index.php/O2k-Chamber_MitoPedia#Leaky_chamber_test Leaky chamber test]«
+:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you change the [[Smoothing|slope smoothing]] to 20 while running experiments with biological sample?'''
+::::::: While running your experiment, especially during [[uncoupler titrations]], it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - »[[Smoothing#DatLab_oxygen_flux:_performance_and_data_analysis|Slope smoothing]]«